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     Current Research Journal of Biological Sciences

    Abstract
2012(Vol.4, Issue:2)
Article Information:

Evaluation of Water and Ethanol Extract of Eucalyptus globulus Labillardiere (Myrtaceae) Leaves against Immature Stages of Filarial Vector Culex quinquefasciatus Say (Diptera: Culicidae)

Angesom Tesfay, Azmera W/Yowhanns, Endahafit Niguse, Guesh Brhane, Selamawit Brhane and Raja Nagappan
Corresponding Author:  Raja Nagappan 
Submitted: 2011 December, 16
Accepted: 2012 January, 13
Published: 2012 March, 10
Abstract:
Aim of the present study was to evaluate larvicidal and pupicidal effect of aqueous and ethanol extract of Eucalyptus globulus leaves against immature stages of Culex mosquitoes. The experiment was conducted in the Botany Laboratory, University of Gondar, Gondar from February 2011 to May 2011. The standard WHO protocol was used to check five concentrations such as 100, 250, 500, 750 and 1000 ppm against II, III, IVth instar larvae and pupae of Culex mosquitoes, respectively. Irrespective of the concentrations tested and the period of exposure, 100% larval mortality was recorded in 1000 ppm concentration after 72 h exposure period followed by 96% in 750 ppm and 90% in 500 ppm. The percentage of larval mortality was based on concentration of the extract and the exposure period. When both are increased the percentage of larval mortality was also increased. After 72 h, 100% pupal mortality was recorded in 500, 750 and 1000 ppm concentration. Study reveled that aqueous and ethanol extract of Eucalyptus leaves is having strong insecticidal activity and it could be useful for the control of larval and pupal stage of Culex mosquitoes in small breeding places. Further, effective simple formulation and field evaluation of this plant extract is suggested for successful implementation of large scale use in mosquito control program.

Key words:  Aqueous, Culex, ethanol, extract, Eucalyptus globulus, larvicidal, pupicidal
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Cite this Reference:
Angesom Tesfay, Azmera W/Yowhanns, Endahafit Niguse, Guesh Brhane, Selamawit Brhane and Raja Nagappan, . Evaluation of Water and Ethanol Extract of Eucalyptus globulus Labillardiere (Myrtaceae) Leaves against Immature Stages of Filarial Vector Culex quinquefasciatus Say (Diptera: Culicidae). Current Research Journal of Biological Sciences, (2): 220-224.
ISSN (Online):  2041-0778
ISSN (Print):   2041-076X
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