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Article Information:
Nodulation Efficiency in Term of Nitrogenase Activity of Rhizobium Mutants and their Wild Type
Neeraj, S.S. Gaurav, S.C. Chatterjee and M.C. Sachin
Corresponding Author: NEERAJ
Submitted: 2009 June, 08
Accepted: 2009 August, 01
Published: |
Abstract:
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This study aimed to select suitable strains that can be used as inoculants to enhance legume
production and simultaneously reduce the use of inorganic fertilizers. It benefited growth of V. radiata and it
was more easily cultured on solid and liquid media than any of the other strains tested including R0132
(1112)::Tn5, R0132 (0097)::Tn5 and R0132 (1106)::Tn5. Mutant R0132 (1106)::Tn5 exhibited superior growth
promoting ability under extreme environmental condition; therefore it has potential to be used in India. A slow
growing nitrogen-fixing strain of Vigna radiata (mung bean), Rhizobium fredii which expressed nitrogenase
activity in a synthetic medium was isolated from its native population. Mutants with decreased and increased
nitrogenase activity were derived from this strain by Tn5 mutagenesis. These mutants were tested for in vivo
symbiotic activity. The efficiency of mutants R0132 (1106)::Tn5 with increased nitrogenase activity in the
culture medium was higher than the parent strain (R0132); This suggests that the plant is perhaps a limiting
factor in the full expression of rhizobial nitrogenase in the nodules. Currently involved in researen aimed at
improving symbiotic nitrogen fixation (SNF) and root nodule sustainability in Vigna radiate. L.
Key words: Nitrogenase activity, symbiotic activity, inoculation, mutant strains, nodulation, nitrogen fixation,
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Cite this Reference:
Neeraj, S.S. Gaurav, S.C. Chatterjee and M.C. Sachin, . Nodulation Efficiency in Term of Nitrogenase Activity of Rhizobium Mutants and their Wild Type. Asian Journal of Agricultural Sciences, (2): Page No: 29-31.
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ISSN (Online): 2041-3890
ISSN (Print): 2041-3882 |
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