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Abstract
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Article Information:
Optimization of Liquid Fermentation Process for Improved Exo-Polysaccharides Production by Kombucha ZJU1
Yan Wu, Qihe Chen, Hui Ruan and Guoqing He
Corresponding Author: Guoqing He
Submitted: September 25, 2012
Accepted: November 13, 2012
Published: February 15, 2013 |
Abstract:
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In order to improve exo-polysaccharides production by Kombucha ZJU1, the optimization of liquid fermentation process for cell growth and Exo-Polysaccharide (EPS) production by Kombucha ZJU1 was conducted with Response Surface Methodology (RSM). A Fractional Factorial Design (FFD) and Central Composite Design (CCD) were applied to optimize the main factors that affect EPS production during fermentation. Both the concentration of brown sugar and inoculation level significantly affected EPS production by Kombucha ZJU1 through FFD experiment. The optimized fermentation process was derived from the predicted model using central composite design as follows: 280 g/L brown sugar, 4 g/L Pu-erh tea, 1 g/L KH2PO4 and 1 g/L (NH4)2 SO4 initial pH unadjusted, the culture medium was inoculated with 1.95% (v/v) Kombucha ZJU1, the cell age was 5 days, then incubated at a temperature of 30±1°C for 6 days on the rotary shaker. The predicted maximal EPS concentration was 2.66 g/L using this optimized fermentation process, which was of value to further work.
Key words: Exo-Polysaccharide (EPS), fermentation process optimization, kombucha, Response Surface Methodology (RSM), , ,
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Cite this Reference:
Yan Wu, Qihe Chen, Hui Ruan and Guoqing He, . Optimization of Liquid Fermentation Process for Improved Exo-Polysaccharides Production by Kombucha ZJU1. Advance Journal of Food Science and Technology, (02): 217-224.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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