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Abstract
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Article Information:
Malaria-Induced Apoptosis in the Cells of Interlukine-6Rα knockout Mice
Saad Hussin Alkahtani
Corresponding Author: Saad Alkahtani
Submitted: April 12, 2012
Accepted: April 25, 2012
Published: July 10, 2012 |
Abstract:
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The purpose of this study was to illustrate the function of IL-6Rα during malaria infection by
investigating the responses of Plasmodium chabaudi malaria in hepatocytes and spleenocytes of IL-6Rα+/+ and
IL-6Rα-/- female C57BL/6 mice via evaluation of mRNA expression of apoptotic genes (Bax, Bcl-2 and
Caspase-3). Mice were injected intraperitoneally (ip) with 106 P. chabaudi-infected erythrocytes and then
scarified at days (0, 1, 4 and 8, respectively). RT-PCR was used to quantify apoptotic genes level. In the IL-
6Rα+/+ the levels of liver Bax were significantly increased at days 1 and 8 while they were significantly
decreased only at day 1 in the IL-6Rα--/- compared with day 0. In contrast, the IL-6Rα+/+ levels of spleen Bax
were significantly increased only at day 8. In the IL-6Rα+/+ the levels of liver Bcl-2 were significantly increased
at all days after infection while significantly decreased only at day 1 in the spleen IL-6Rα-/- compared with
day 0. In the IL-6Rα+/+ the levels of liver caspase-3 were significantly increased at days 1 and 8 while
significantly decreased in the IL-6Rα-/- at day 8 compared with day 0. However, in the IL-6Rα+/+ the levels
of spleen caspase-3 were significantly increased at day 8 only while significantly decreased at day 8 in the IL-
6Rα-/-compared with day 0. In conclusion, this data has shown that the absence of IL-6Rα plays an important
role in decreasing the severity of apoptosis during malaria disease. Thus, this study suggests that after further
investigation the role of IL-6Rα could be the therapeutic key for determining the outcome of malaria in liver
cells.
Key words: Apoptosis, IL-Rα and spleen, liver, malaria, mice, ,
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Abstract
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Cite this Reference:
Saad Hussin Alkahtani, . Malaria-Induced Apoptosis in the Cells of Interlukine-6Rα knockout Mice. Current Research Journal of Biological Sciences, (4): 500-506.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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