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Abstract
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Article Information:
Micro-Propagation of Vanilla planifolia Using Enset (Ensete ventricosum (Welw, cheesman)) Starch as a Gelling Agent
Ayelign Mengesha, Biruk Ayenew, Elias Gebremariam and Tewodros Tadesse
Corresponding Author: Ayelign Mengesha
Submitted: May 09, 2012
Accepted: June 01, 2012
Published: July 10, 2012 |
Abstract:
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Vanilla planifolia micro-propagation efficiency was evaluated using enset starch (bulla) as an
alternative gelling agent. Particularly, optimization of bulla concentration levels and assessment of costs
saved using bulla were carried out in this study since in vitro media ingredient, especially agar, comprises
of the highest cost as compared to others ingredients. The results revealed that shoot numbers and fresh
weight were significantly improved in multiplication media gelled with bulla (8%). Although all tested
gelling agents apparent 100% rooting, increased newly growth of shoots was obtained on media
supplemented with bulla (8%) followed by bulla (10%) and agar. Substitution of agar with bulla (8%) could
save about 72.5% of agar cost per a litter media. Generally, replacing agar with 6-10% bulla could decline
the cost of agar about 50-72.5% per a litter media. However, bulla resulted in poor media clarity that cause
problem in contamination detection and enhanced root proliferation during multiplication phase. Hence,
bulla seems to be more preferable in rooting phase that needs short growth period and improved the
plantlets growth. In the future, detail studies would be needed on characterization of bulla starch to assure
the principal active ingredients depending upon source of cultivars and locations as well as to assess effects
on different crops.
Key words: Alternative gelling agent, bulla starch, cost reduction, in vitro propagation, vanilla, ,
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Cite this Reference:
Ayelign Mengesha, Biruk Ayenew, Elias Gebremariam and Tewodros Tadesse, . Micro-Propagation of Vanilla planifolia Using Enset (Ensete ventricosum (Welw, cheesman)) Starch as a Gelling Agent. Current Research Journal of Biological Sciences, (4): 519-525.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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