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Abstract
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Article Information:
Callus Induction of Ocimum sanctum and Estimation of Its Total Flavonoids Content
Zi Xiong Lim, Anna Pick Kiong Ling and Sobri Hussein
Corresponding Author: Anna Pick Kiong Ling
Submitted: 2009 July, 14
Accepted: 2009 July, 28
Published: |
Abstract:
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Ocimum sanctum, also known as “H oly Basil” is one of the most common medicinal plants used by
diverse cultures and tribal groups. Callus induction from leaf explants of O. sanctum was conducted by
incubating leaf explants on Murashige and Skoog (MS) medium supplemented with 2, 4!dichlorophenoxyacetic
acid (2,4!D), picloram, and indole-butyric acid (IBA) at 0, 1, 3, and 5 mg/L as well as the combination of 3
mg/L picloram with different concentrations (0, 0.5, 1.0, 1.5, and 2.0 m g/L) of 6!benzylaminopurine (BAP)
or kinetin. Results obtained from the studies revealed that all the leaf explants incubated on phytohormonesupplemented
medium formed callus. Leaf explants grown on 3 mg/L picloram formed callus after 8±1 days
of culture, and degree of callus formation w as found to be the highest (++++) among all the single auxin
treatments. In contrast, the degree of callus formed from the leaf explants cultured on MS medium
supplemented with combination of auxin and cytokinins were evidently lower than those in the single auxin
treatments. Leaf explants cultured on kinetin-supplemented MS medium showed a higher degree of callus
formation (++++) as compared to BAP-supplemented MS medium. There was no significant difference between
the days of callus formation among all the cytokinin-supplemented treatments. The total flavonoids content of
leaf-derived callus cultured on 3 mg/L picloram w ere also estimated and compared with the in vivo leaf tissues.
The aluminium (III) chloride colorimetric assay revealed that the total flavonoids content of in vivo leaf tissues
of O. sanctum were 2.2 times higher than leaf-derived callus, whereby the former produced 0.733±0.077
miligram catechin equivalent per gram fresh weight (mg CE/g fresh weight), while the latter only yielded
0.333±0.043 mg CE/g fresh weight.
Key words: Anti-oxidant, essential oil, Holy Basil, Ocimum sanctum, orientin, vicenin,
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Cite this Reference:
Zi Xiong Lim, Anna Pick Kiong Ling and Sobri Hussein, . Callus Induction of Ocimum sanctum and Estimation of Its Total Flavonoids Content. Asian Journal of Agricultural Sciences, (2): Page No: 55-61.
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ISSN (Online): 2041-3890
ISSN (Print): 2041-3882 |
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