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Abstract
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Article Information:
Efficient Nitrogen Fixing Rhizobial Isolate Infecting Vigna radiata L.
Neeraj, S.S. Gaurav, S.C. Chatterjee ,Sachin and Mahesh Chandra
Corresponding Author: Neeraj Tandan
Submitted: 2009 August, 09
Accepted: 2009 August, 28
Published: |
Abstract:
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Among 972 rhizobia, isolated from nodules of different verities of Vigna radiata, 67 isolates were
found to be potential ones on the basis of plant growth promotion/ increased plant biomass. These isolates w ere
further screened, where, only six potential isolates, showed higher plant biomass, maximum nodule numbers
and higher nitrogenase activity, were selected for further studies. Maximum range of fresh weights of nodule
per plant w as shown again by isolate R0132 and noticed to be 261.37 mg nodule/plant. Nitrogenase activity
of plant nodule inoculated with different rhizobial isolates varied from 2.56 to 3.21 :g/mg/nodule. When
growth abilities of these isolates studied, it had been observed that with different nitrogen sources, isolate
R0132 was found to be the best candidate as it utilized a wide range of nitrogen and carbon sources and was
able to grow at slightly higher temperature i.e. 32ºC and slightly acidic (pH 6.0) and alkaline (pH 8.0)
conditions. Furthermore, this isolate demonstrated the ability to grow in presence 50mM concentration of NaCl.
Key words: Vigna radiata, nitrogen fixation, nitrogenase activity, rhizobial isolates., , ,
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Cite this Reference:
Neeraj, S.S. Gaurav, S.C. Chatterjee ,Sachin and Mahesh Chandra, . Efficient Nitrogen Fixing Rhizobial Isolate Infecting Vigna radiata L.. Asian Journal of Agricultural Sciences, (2): Page No: 62-65.
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ISSN (Online): 2041-3890
ISSN (Print): 2041-3882 |
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