Abstract

Studying of genetic relationships among Aegilops L. and Triticum L. species by direct sequencing of PCR-amplified Internal Transcribed Spacer (ITS) of nuclear ribosomal DNA to investigate the polymorphism in nucleotide sequences among 19 Aegilops L. and 7 Triticum L. species. ITS sequences were aligned with CLUSTAL W 2.1 multiple sequence aligment program. The phylogenetic relationships among species were reconstructed using Unweighted Pair Group Mean Arithmetic average (UPGMA) and Neighbor-Joining (NJ) methods. ITS region ranged from 600 to 602 bp. The length of ITS1 was 221-222 bp and ITS2 was 215-217 bp. The 5.8S subunit was 163 bp long. The G+C content of the ITS1 region ranged from (60.3 to 63.9) % in all Triticum and Aegilops species. The G+C content of the 5.8S subunit was entirely identical in all species, 59.5%. The G+C content of the ITS2 region ranged from 59.9 to 63.5%. There were 67 variable sites (11.12%) in the entire ITS region. The phylogenetic relationships among species were reconstructed using Unweighted Pair Group Mean Arithmetic average (UPGMA) were discussed.

Keywords:

Aegilops, genetic relationships, internal transcribed spacer, ITS, Triticum,

References

Competing interests

The authors have no competing interests.

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The authors have no competing interests.