Abstract

In this study, response surface methodology was employed to optimize the extraction process of Crude Polysaccharides from Chlorella pyrenoidosa (CCPS) on the activity of saccharase with maximum inhibition. Three independent and main variables, including extraction time (h), temperature (C) and ratio of liquid to raw material (mL/g), which were of significance for the saccharase inhibition rate of CCPS were studied and the quadratic regression rotary combination design was based on the results of a single-factors test. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis and also examined using the appropriate statistical methods. The best extraction conditions are as follows: extraction time 100 min, temperature 85C, ratio of liquid to raw material 50 mL/g. Under the optimization conditions, the experimental saccharase inhibition rate of CCPS was 42.27%, which was well matched with the predictive inhibition rate of 41.78% and the yield of polysaccharide was 4.23%.

Keywords:

Chlorella pyrenoidosa, polysaccharides, response surface methodology, saccharase,

References

1. Cherng, J.Y. and M.F. Shih, 2006. Improving glycogenesis in streptozocin (STZ) diabetic mice after administration of green algae chlorella. Life Sci., 78(11): 1181-1186.
   CrossRef    PMid:16289560    
2. Kay, R.A., 1991. Microalgae as food and supplement. Crit. Rev. Food Sci., 30(6): 555-573.
   CrossRef    PMid:1741951    
3. Miyazawa, Y., T. Murayama, N. Ooya, L.F. Wang, Y.C. Tung and N. Yamaguchi, 1988. Immunomodulation by a unicellular green algae (Chlorella pyrenoidosa) in tumor-bearing mice. J. Ethnopharmacol., 24(2-3): 135-146.
   CrossRef    
4. Robledo, D. and Y.F. Pelegrin, 1997. Chemical and mineral composition of six potentially edible seaweed species of Yucatan. Bot. Mar., 40: 301-306.
   CrossRef    
5. Senthilkumar, T. and N. Ashokkumar, 2012. Impact of Chlorella pyrenoidosa on the attenuation of hyperglycemia-mediated oxidative stress and protection of kidney tissue in streptozotocin-cadmium induced diabetic nephropathic rats. Biomed. Prev. Nutr., 2(2): 125-131.
   CrossRef    
6. Sheng, J.C., F. Yu, Z.H. Xin, L.Y. Zhao, X.J. Zhu and Q.H. Hu, 2007. Preparation, identification and their antitumor activities in vitro of polysaccharides from Chlorella pyrenoidosa. Food Chem., 105: 533-539.
   CrossRef    
7. Shi, Y., J.C. Sheng, F.M. Yang and Q.H. Hu, 2007. Purification and identification of polysaccharide derived from Chlorella pyrenoidosa. Food Chem., 103: 101-105.
   CrossRef    
8. Suárez, E.R., J.A. Kralovec, M.D. Noseda, H.S. Ewart, C.J. Barrow, M.D. Lumsden and T.B. Grindley, 2005. Isolation, characterization and structural determination of a unique type of arabinogalactan from an immunostimulatory extract of Chlorella pyrenoidosa. Carbohyd. Res., 340: 1489-1498.
   CrossRef    PMid:15882847    
9. Suárez, E.R., J.A. Kralovec and T.B. Grindley, 2010. Isolation of phosphorylated polysaccharides from algae: The immunostimulatory principle of Chlorella pyrenoidosa. Carbohyd. Res., 345: 1190-1204.
   CrossRef    PMid:20466355    
10. Yang, F.M., Y. Shi, J.C. Sheng and Q.H. Hu, 2006. In vivo immunomodulatory activity of polysaccharides derived from Chlorella pyrenoidosa. Eur. Food Res. Technol., 224(2): 225-228.
    CrossRef    
11. Zhu, C.P. and X.L. Liu, 2013. Optimization of extraction process of crude polysaccharides from pomegranate peel by response surface methodology. Carbohyd. Res., 92: 1197-1202.
    CrossRef    PMid:23399146    

Competing interests

The authors have no competing interests.

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