Research Article | OPEN ACCESS
Bioactivity-guided Isolation and Characterization of Antitumor Active Compound from Marine-derived Fungus Penicillium chrysogenum HGQ6
1, 2Lei Guo, 1Tao Huang, 1Bing Hu and 3Cong Wang
1Jiangsu Institute of Marine Resources
2School of Ocean, Huaihai Institute of Technology, Lianyungang 222005
3Key Laboratory of Marine Drugs, Ministry of Education of China, Ocean University of China, Qingdao 266003, China
Advance Journal of Food Science and Technology 2016 5:353-355
Received: May 16, 2015 | Accepted: June 22, 2015 | Published: February 15, 2016
Abstract
To isolate and characterize antitumor active compounds (bioactivity-guided fractionation) from the ethyl acetate extract of fermentation broth of marine-derived fungus Penicillium chrysogenum HGQ6. Following the cytotoxicity against human cancer cell line BGC823, one compound was isolated and purified by silica gel chromatography, Sephadex LH-20 chromatography and preparative high performance liquid chromatography. The isolated compound was elucidated on the basis of extensive spectroscopic data analysis as 5-hydroxymethyl-2-furancarboxaldehyde, which exhibited cytotoxicity against BGC823 cell line with an IC50 value of 0.19 mg/mL. Our present study concluded that, 5-Hydroxymethyl-2-furancarboxaldehyde isolated from marine-derived fungus Penicillium chrysogenum HGQ6 has a certain extent of antitumor potential.
Keywords:
Antitumor activity, isolation and characterization, marine-derived fungus, Penicillium chrysogenum HGQ6,
References
- Gao, S.S., X.M. Li, F.Y. Du, C.S. Li, P. Proksch and B.G. Wang, 2011. Secondary metabolites from a marine-derived endophytic fungus Penicillium chrysogenum QEN-24S. Mar. Drugs, 9(1): 59-70.
CrossRef PMid:21339945 PMCid:PMC3039470 -
Guo, L., J.H. Lian, W. Ji, W.R. Hu, G.L. Wu and B.Q. Gong, 2006. Establishment of a cell-based drug screening system for identifying selective down-regulators of mPGES-1. Inflamm. Res., 55(3): 114-118.
CrossRef PMid:16673154
-
Guo, L., W. Zhu and C. Liu, 2012. Fermentation conditions optimization of anti-tumor active metabolites from marine Penicillium sp. HGQ6. Adv. Mater. Res., 340: 344-350.
- Guo, L., W. Zhu and A. Zheng, 2014. Identification and fermentation optimization of an antibacterial Aspergillus tubingensis associated with the crab Portunus triuberbuculatus. J. Pure Appl. Microbiol., 8(2): 1627-1633.
-
Millward, M., P. Mainwaring, A. Mita, K. Federico, G.K. Lloyd, N. Reddinger, S. Nawrocki, M. Mita and M.A. Spear, 2012. Phase 1 study of the novel vascular disrupting agent plinabulin (NPI-2358) and docetaxel. Invest. New Drug., 30(3): 1065-1073.
CrossRef PMid:21327495
- Peng, X., Y. Wang, K. Sun, P. Liu, X. Yin and W. Zhu, 2011. Cerebrosides and 2-pyridone alkaloids from the halotolerant fungus Penicillium chrysogenum grown in a hypersaline medium. J. Nat. Prod., 74(5): 1298-1302.
CrossRef PMid:21381678
-
Pyo, M.K., J.L. Jin, Y.K. Koo and H.S. Yun-Choi, 2004. Phenolic and furan type compounds isolated from Gastrodia elata and their anti-platelet effects. Arch. Pharm. Res., 27(4): 381-385.
CrossRef PMid:15180301
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
|
|
![](http://www.maxwellsci.com/images/AJFST-maxw.jpg) |
ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
![Submit Manuscript](../images/sub.jpg) |
Information |
|
|
|
Sales & Services |
|
|
|