Research Article | OPEN ACCESS
Effect of Different Ratio of Cake Fertilizer and Inorganic Fertilizer on Free-amino Acids in Burley Leaves
1Jinfeng Zhu, 2Yuzhen Gao, 3Weifeng Wang, 4Ping Lu, 4Yuehua Zhang, 5Min Xu and 6Yanqiu Jing
1Luohe of Henan National Tobacco Corporation, Luohe 462000
2Tobacco Industry Co., Ltd., Technology Research and Development Center in Gansu Province, Lanzhou 730050
3Guangxi Zhuang Autonomous Region Provincial Branch of CNTC; Nanning, 530022
4Chinese Tobacco Industrial Company in Henan, Zhengzhou 450000
5China National Tobacco Corporation of Henan Province
6Henan Agricultural University/National Tobacco Cultivation and Physiology and Biochemistry Research Centre/Key Laboratory for Tobacco Cultivation of Tobacco Industry, Zhengzhou 450002, China
Advance Journal of Food Science and Technology 2016 6:412-416
Received: May ‎25, ‎2015 | Accepted: June ‎22, ‎2015 | Published: February 25, 2016
Abstract
A Reversed-Phase High Performance Liquid Chromatographic method was developed for determining free Amino Acids in Burley Tobacco. The test was done by OPA/3-mercaptopropionic Acid as the pre-column derivatising reagent. Chromatographic column was Elitte C18 column (4.6 mm×250 mm i.d., 5 um). Mobile phase A was 18 mol/L NaAc (PH7.2) including 0.002% (v/v) trimethylamine and 0.3% (v/v) furanidine. Mobile phase B was 100 mol/L NaAc (PH7.2) -Acetonitrile-methanol (v/v = 1:2:2). The column temperature was 40°C and the flow rate was 1.0 mL/min. The fluorescence detector was used with 350 nm excitation wave length and 450 nm emission wave length. The average recoveries of the method ranged 95.3 to 100.7% with the relative standard deviation of 2.32~9.24%. The method is simple, accurate and has good repeatability. The results of the determination of seventeen kinds of free Amino Acids in burley leaves which were produced by way of different ratio of cake fertilizer and inorganic fertilizer. The results show that Aspartic acid has the highest content however ratio of cake fertilizer and inorganic fertilizer. The contents most of free Amino Acids are increased and then gradually decreased with the increase of organic manure. The contents most of free Amino Acids are very close at 15:85% ratio and 30:70% ratio of cake fertilizer and inorganic fertilizer. The total amount of free Amino Acids is the highest at 30:70% ratio of cake fertilizer and inorganic fertilizer. Considering comprehensively, the quality of burley leaves is the best at 30:70% ratio of cake fertilizer and inorganic fertilizer.
Keywords:
Burley leaves, different ratio of cake fertilizer and inorganic fertilizer, free amino acids,
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Competing interests
The authors have no competing interests.
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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
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