Abstract

The valorization of chicken intestine to formulate a growth medium for lactic acid bacteria was tested on a strain of Lactobacillus brevis CHTD27. For this purpose, an autolytic degradation of the chicken intestine was carried out. The resulted Chicken Intestine Autolysate (CIA) was used as basic medium, while other medium parameters were studied using a Plackett and Burman design to determine the significant factors affecting the strain growth. Those were further optimized using a central composite design. As a result, CIA containing 19.15 g/L of glucose and 0.25 g/L of ammonium citrate supported good growth of L. brevis with a final optical density value of 6.00 at 600 nm, which is 32% higher than that on the standard medium MRS. Thus, medium containing CIA could constitute a low-cost alternative to MRS medium for the production of L. brevis biomass.

Keywords:

Autolysis, chicken intestine, growth medium, Lactobacillus brevis, statistical optimization,

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