Abstract

Using Yam starch as raw material, the fermentation technology of Single Cell Protein (SCP) was optimized through by active dry yeast fermentation through single factor and orthogonal test in this study. The optimal conditions were showed as followed: 30 mL the medium volume in 250 mL flask, inoculum size17%, fermentation time 69 h, pH 5.0. Under these conditions, the production of SCP reached 241.54±0.15 (g wet weight/100 g Dry starch.

Keywords:

Active dry yeast, orthogonal test, SCP, yam starch,

References

1. Anupama, X. and P. Ravindra, 2000. Value-added food: Single cell protein. Biotechnol. Adv., 18: 459-479.
   CrossRef    
2. Bertrand, J., B. Liagre, G. Bégaud-Grimaud, M.O. Jauberteau, J.L. Beneytout, P.J.P. Cardot and S. Battu, 2009. Analysis of relationship between cell cycle stage and apoptosis induction in K562 cells by sedimentation field-flow fractionation. J. Chromatogr. B., 877(11-12): 1155-1161.
   CrossRef    PMid:19299207    
3. Buschke, N., R. Schafer, J. Becker and C. Wittmann, 2013. Metabolic engineering of industrial platform microorganisms for biorefinery applications-optimization of substrate spectrum and process robustness by rational and evolutive strategies. Bioresource Technol., 135: 544-554.
   CrossRef    PMid:23260271    
4. Chanda, S. and S. Chakrabarti, 1996. Plant origin liquid waste: a resource for single cell protein production by yeasts. Bioresource Technol., 57: 51-54.
   CrossRef    
5. Chen, G.T., M. Yang, Y. Song, Z.Q. Lu, J.Q. Zhang, H.L. Huang, S.H. Guan, L.J. Wu and D.A. Guo, 2008. Comparative analysis on microbial and rat metabolism of ginsenoside Rb-1 by high-performance liquid chromatography coupled with tandem mass spectrometry. Biomed. Chromatogr., 22(7): 779-785.
   CrossRef    PMid:18384066    
6. Cheng, P., H.Z. Zhao, B. Zhao and J.R. Ni, 2009. Pilot treatment of wastewater from Dioscorea zingiberensis C.H. Wright production by anaerobic digestion combined with a biological aerated filter. Bioresource Technol., 100(12): 2918-2925.
   CrossRef    PMid:19268578    
7. Guo, X.S. and M. Xiao, 2006. The development prospects and application of single cell protein. China's Food Nutr., 5: 24-251.
   
8. Huang, W., H.Z. Zhao, J.R. Ni, H. Zuo, L.L. Qiu and H. Li, 2008. The best utilization of D. zingiberensis C. H. Wright by an eco-friendly process. Bioresource Technol., l99: 7407-7411.
   CrossRef    PMid:18321698    
9. Jayachandran, K.S., H.R. Vasanthi and G.V. Rajamanickam, 2009. Antilipoperoxidative and membrane stabilizing effect of diosgenin, inexperimentally induced myocardial infarction. Mol. Cell. Biochem., 327: 203-210.
   CrossRef    PMid:19234676    
10. Kosseva, M.R., 2013. Recovery of Commodities from Food Wastes using Solid-state Fermentation. In: Kosseva, M.R. and C. Webb (Eds.), Food Industry Wastes: Assessment and Recuperation of Commodities. Academic Press, Elsevier, Amsterdam, pp: 77-102.
    CrossRef    
11. Liang, X.J., H. Hu, C. Chang and J. Wang, 2003. Study on the technology of producing alcohol with wheat starch by TH-AADY. Liquor-Making Sci. Technol., 4: 62-63.
    
12. Ma, Y., L.M. Zhang and Y. Yuan, 2006. Study on physicochemical properties of yam starch. Sci. Technol. Food Ind., 27(7): 75-77.
    
13. Nasseri, A.T., S. Rasoul-Amini, M.H. Moromvat and Y. Ghasemi, 2011. Single cell protein: Production and process. Am. J. Food Technol., 6: 103-116.
    CrossRef    
14. Nigam, J.N., 1998. Single cell protein from pineapple cannery effluent. World J. Microb. Biot., 14: 693-696.
    CrossRef    
15. Rajoka, M.I., M.A.T. Kiani, S. Khan, M.S. Awan and A.S. Hashmi, 2004. Production of single cell protein from rice polishings using Candida utilis. World J. Microb. Biot., 20: 297-301.
    CrossRef    
16. Ravindra, A.P., 2000. Value-added food: Single cell protein. Biotechnol. Adv., 18: 459-479.
    CrossRef    
17. Ren, J.B., 2007. The development and utilization of single cell protein feed. Feed Nutr., pp: 31-321.
    
18. Trouillas, P., C. Corbiere, B. Liagre, J.L. Duroux and J.L. Beneytout, 2005. Structure-function relationship for saponin effects on cell cyclearrest and apoptosis in the human 1547 osteosarcoma cells: a molecular modeling approach of natural molecules structurally close to diosgenin. Bioorg. Med. Chem. Lett., 13: 1141-1149.
    CrossRef    PMid:15670922    
19. Wang, Y.X., H. Liu, J.G. Bao, Y. Hong, Z.H. Yang and C.X. Zhang, 2008. The Saccharification-Membrane Retrieval-Hydrolysis (SMRH) process: A novel approach for cleaner production of diosgenin derived from Dioscorea zingiberensis. J. Clean. Prod., 16: 1133-1137.
    CrossRef    
20. Wijeyaratne, S.C. and A.N. Jayathilake, 2000. Characteristics of two yeast strains (Candida tropicalis) isolated from Caryota urens (Kithul) toddy for single cell protein production. J. Natl. Sci. Found Sri Lanka, 28: 79-86.
    
21. Zhao, X., X.J. Xie, S.T. Chen and Z.H. Yang, 2004. Technique and countermeasure of dealing with wastewater of D. zingiverensis Wright industry in the source water of South-water-to-north project. Safety Environ. Eng., 11: 14-17.
    

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