Research Article | OPEN ACCESS
Analysis of ${NO_2}^-$ and ${NO_3}^-$ in Apples, Pears and Oranges with Ion Chromatography
1, 2Dongwu Liu, 2Hong Guo, 1Ling Kong and 1Zhiwei Chen
1School of Life Sciences,
2Analysis and Testing Center, Shandong University of Technology, 255049, Zibo, China
Advance Journal of Food Science and Technology 2016 1:49-53
Received: June ‎8, ‎2015 | Accepted: July ‎8, ‎2015 | Published: May 05, 2016
Abstract
Ion Chromatography (IC) can yield the precise and reproducible data if the experimental condition is kept constant. In this study, the anions ${NO_2}^-$ and ${NO_3}^-$ in apples, pears and oranges were determined with the technique of IC. A Dionex ICS-2000 ion chromatograph with a Dionex gradient pump, eluent degassing module and conductivity detector was used to detect ${NO_2}^-$ and ${NO_3}^-$. Anions were separated on a DIONEX Ionpac AS19 A-4 mm ion-exchange column, with an Iopac AG AS19 A-4 mm guard column and detected after suppression with ASRS 300 anion electrical self-regenerating suppressor. The results indicated that the technique of IC was suitable for the rapid, precise and accurate determination of ${NO_2}^-$ and ${NO_3}^-$ in apples, pears and oranges. In addition, the acceptable detection limits were obtained for ${NO_2}^-$ and ${NO_3}^-$ and the analysis time was significantly shortened with the technique of IC. The data will provide theories and rapid methods for the supervision of fruit quality.
Keywords:
Apple, ion chromatography, ${NO_2}^-$ and ${NO_3}^-$, orange, pear,
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