Research Article | OPEN ACCESS
Bioassay-guided Separation and Identification of Pharmacodynamic Constituents with Nitrite-scavenging Effect in Aquilaria sinensis Leaves
1, 2Yang Maoxun, 1, 3Chen Heru, 4Huang Yongfang and 2Zhao Bin
1Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632
2Zhongshan Torch Polytechnic, Zhongshan 528436
3Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Guangzhou 510632
4College of Forestry, South China Agricultural University, Guangzhou 510642, China
Advance Journal of Food Science and Technology 2016 5:416-419
Received: August ‎24, ‎2015 | Accepted: September ‎11, ‎2015 | Published: June 15, 2016
Abstract
To explore the pharmacodynamic constituents with nitrite-scavenging effect in Aquilaria sinensis leaves, a new food raw material, applying bioassay-guided method by using UV spectrophotometry, scavenging rates of crude extracts of wild and cultivated A. leaves with different solvents, each polar fraction of the crude extracts and each cut fractions from the n-butanol fraction were tested. It is disclosed that scavenging effect of the crude extract from wild A. leaves was better than that from cultivated. n-Butanol fraction of the crude extract from wild A. with 70% acetone displayed the best scavenging effect among all the counterparts. Mangiferin (1) and 2-O-&alpha-L-rhamnopyranosyl-4, 6, 4'-trihydroxybenzophenone (2) were identified as the two most active compounds with scavenging rate up to 29.4±0.7% and 24.6±0.8%, at the conditions of pH 3.0, 37°C and 30 min treatment, respectively.
Keywords:
Aquilaria sinensis, bioassay-guided method, mangiferin, nitrite,
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