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     Advance Journal of Food Science and Technology

Research Article   |   OPEN ACCESS

Development of an IMB-ELISA Method for Analyzing Pseudomonas fluorescens

Wei Yang and Xianjun Dai
College of Life Science, Key Laboratory of Marine Food Quality and Hazard Controlling Technology, Hangzhou, Zhejiang Province, China
Advance Journal of Food Science and Technology  2016  12:765-769
http://dx.doi.org/10.19026/ajfst.11.2789  |  © The Author(s) 2016
Received: April ‎7, ‎2015  |  Accepted: April ‎22, ‎2015  |  Published: August 25, 2016
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Abstract

To develop an immunemagnetic-bead-based enzyme-linked immunesorbent assay (IMB-ELISA), antisera were prepared12 from New Zealand white rabbits immunized with inactivated Pseudomonas fluorescens (P. fluorescens) and conjugated with magnetic-bead to get immunomagnetic-beads. IMB-ELISA would be developed through IMB collecting P. fluorescens. The results showed that the limit of detection ELISA and IMB-ELISA are, respectively 101.1 cfu/mL and 101.0 cfu/mL. The IMB-ELISA is more sensitive and higher efficient than IC-ELISA by calculating the recovery rate and the coefficient of variation, because of IMB quickly enriching the target bacteria. The IMB-ELISA is a quick and accurate method for the detection of P. fluorescens.

Keywords:

ELISA, IMB, Pseudomonas fluorescens,

References