Abstract

To enhance carotenoids production from Rhodobacter sphaeroides, Nitrosoguanidine (NTG) mutagenesis was employed to construct high-carotenoids Rb. Sphaeroides strain in the present study. Experimental results suggested that the NTG concentration and treat duration was 3 mg/mL and 15 min, respectively. One high-carotenoids Rb. Sphaeroides strain was finally obtained and the production of carotenoids was 2 times higher than that of the production from wild type Rb. Sphaeroides. The present study promotes to large scale production of natural carotenoids from Rb. Sphaeroides.

Keywords:

Carotenoids, mutagenesis, NTG, Rhodobacter sphaeroides,

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