Abstract

In this study, antioxidant effects in vitro and in vivo by the Caenorhabditis elegans model of Auricularia auricula polysaccharides (AAP1, AAP2 and AAP3) were used to compare these three kinds of polysaccharide extraction processes (hot water extraction, enzyme extraction and alkali extraction). And the monosaccharide composition of these polysaccharides was determined by Gas Chromatography (GC) method. Results suggested that AAP2, which was mainly composed of arabinose, D-xylose, 2-Deoxy-D-arabino-hexose, mannose, glucose and galactose with the molar ratio of 1:0.44:0.33:1.67:1:0.17, possessed stronger antioxidant activity. It could scavenge free radicals, up-regulation of antioxidant enzyme (superoxide dismutase (SOD) by 91.25% and catalase (CAT) by 36.67%, compared with control). It could also reduce the level of Reactive Oxygen Species (ROS) in C. elegans under oxidative stress. In conclusion, enzyme extraction is better in preparing AAP.

Keywords:

Auriculaia auricula polysaccharide, antioxidant activity, extraction processes,

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