Abstract

To reveal the relationship between theγ-Glutamyltranspeptidase (GGT) and endogenous formaldehydein shiitake mushroom based on gene level, we cloned the GGT gene from shiitake mushroom. The full-length sequence is 2063 bp contained a 5’-terminal Untranslated Region (UTR) of 36 bp, a 3’-terminal UTR of 113 bp and an ORF of 1914 bp encoding a polypeptide of 637 amino acids, which is high conserved in fungus. Quantitative real-time RT-PCR revealed the change of GGT expression along the growing process of shiitake mushroom, which increased first and then decreased (p<0.05). Simultaneously, the GGT activity and endogenous formaldehyde were determined and had the same trend with mRNA expression (p<0.05). In addition, at each growth stage the GGT expression, activity and formaldehyde were higher in stalks than in caps (p<0.05). The results confirmed the positive relationship between GGT and endogenous formaldehyde production, which hinted that endogenous formaldehyde could be controlled by regulating the expression of GGT and activity.

Keywords:

&gamma-Glutamyltranspeptidase, formaldehyde, gene cloning, mushroom,

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