Research Article | OPEN ACCESS
Detection of Cronobacter sakazakii in Powdered Infant Formula using Real-time Fluorescence Single Primer Isothermal Amplification
Yingli Chen, Xiaoyan Ma, Xunzhe Zhang, Yu Wang, Xianzhou Zhang, Wei Zhang and Ning Yuan
College of Food Science and Technology, Agricultural University of Hebei, No. 2596 Lekainan Road, Nanshi District, Baoding, Hebei Province 150090, P.R. China
Advance Journal of Food Science and Technology 2016 12:688-697
Received: February ‎29, ‎2016 | Accepted: May ‎23, ‎2016 | Published: December 25, 2016
Abstract
Specific primers and blockers were designed based on Cronobacter sakazakii gene (16SrRNA) and Real-time Fluorescence Single Primer Isothermal Amplification (RF-SPIA) was established to detect C. sakazakii. This method identified that C. sakazakii genes were positive, whereas other non-C. sakazakii genes were negative. The sensitivity of this test for C. sakazakii pure culture reached 1.4×100CFU/mL and that for C. sakazakii genome reached 7.5×101fg/mL. The detection limit of RF-SPIA in milk powder artificially contaminated with C. sakazakii could reach 8.1×101CFU/mL. These results demonstrate that the RF-SPIA detection method for C. Sakazakii is highly sensitive, strong, specific and convenient.
Keywords:
Cronobacter sakazakii, 16SrRNA, detection, Real-time fluorescence single primer isothermal amplification,
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Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
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The authors have no competing interests.
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