Abstract

The objective of this study was to investigate the inhibitory effect of Polypeptide from Argopecten Irradias (PAI) on mice with transplanted H₂₂ hepatocarcinoma. PAI was prepared by enzymatic hydrolysis and separation and the results of its structure identification indicated that it contained five polypeptide components with molecular weights of 700-1000 Da. Forty Kunming mice were randomly divided into H₂₂ hepatocarcinoma group and low, medium and high dose PAI groups. After ig administration of normal saline and different doses of PAI for 10 days, all mice were inoculated with H₂₂ hepatocarcinoma cell suspension to establish models. After continuous gavage for 10 days, the blood was collected from the eyeball and the mice were sacrificed for index detection. It was found that the tumor inhibiting rates of PAI in three dose groups were 28.16, 41.93 and 84.00%, respectively. Compared to H22 hepatocarcinoma group, the mutant serum p53, survivin and MDA levels of mice in three PAI-dose groups were significantly reduced (p<0.01), the IL-2 and SOD levels were significantly increased (p<0.01). The results suggested that PAI had obvious inhibitory effect on mice with H₂₂ hepatocarcinoma cells.

Keywords:

p53 protein, H₂₂ tumor-bearing mice, polypeptide from Argopecten irradias, survivin protein, tumor inhibiting rate,

References

1. Acun, T., E. Terzioglu-Kara, O. Konu, M. Ozturk and M.C. Yakicier, 2010. Mdm2 Snp309 G allele displays high frequency and inverse correlation with somatic p53 mutations in hepatocellular carcinoma. Mutat. Res., 684(1-2): 106-108.
   CrossRef    PMid:19954744    Direct Link
2. Aneiros, A. and A. Garateix, 2004. Bioactive peptides from marine sources: pharmacological properties and isolation procedures. J. Chromatogr. B., 803(1): 41-53.
   CrossRef    PMid:15025997    Direct Link
3. Chen, H.Y., X. Chu, C.L. Yan, X.H. Chen, M. Sun, Y.J. Wang, C.B. Wang, W.G. Yu, 2007. Polypeptide from Chlamys farreri attenuates murine thymocytes damage induced by ultraviolet B. Acta Pharm. Sin., 28(10): 1665-1670.
   CrossRef    PMid:17883955    Direct Link
4. Collins, A., 2005. Antioxidant intervention as a route to cancer prevention. Eur. J. Cancer, 41(3): 1923-1930.
   Direct Link
5. Ding, B.X. and C.B. Wang, 2003. Inhibitory effect of polypeptides from Chlamys farreri on UVB-induced apoptosis and DNA damage in normal human dermal fibroblasts in vitro. Acta Pharmacol. Sin., 24(10): 1006-1010.
   PMid:14531943    Direct Link
6. Du, W., X.P. Liu, H. Liang, Y.Z. Wang and C.B. Wang, 2000. Analysis on the protective effect of polypeptide from Chlamys farreri on lymphocytes and reversed-phase liquid chromatography. Chin. J. Marine Drug., 19(5): 27-29.
   
7. Fukuda, S. and L.M. Pelus, 2006. Survivin: A cancer target with an emerging role in normal adult tissues. Mol. Cancer Ther., 5(5): 1087-1098.
   CrossRef    PMid:16731740    Direct Link
8. Hoelzl, C., J. Bichler, F. Ferk, T. Simic, A. Nersesyan and L. Elbling, V. Ehrlich, A. Chakraborty and S. Knasmüller, 2005. Methods for the detection of antioxidants which prevent age related diseases: A critical review with particular emphasis on human intervention studies. J. Physiol. Pharmacol., 56(Suppl. 2): 49-64.
   PMid:16077190    Direct Link
9. Hussain, S.P., L.J. Hofseth and C.C. Harris, 2003. Radical causes of cancer. Nat. Rev. Cancer, 3(4): 276-285.
   Direct Link
10. Jin, A.H., L. Pu, X.Z. Yin and J.S. Quan, 2012. The tumor inhibiting effect of iridoid glucosides from Boschniakia rosscica on H22 hepatoma transplanted tumors in mice. Chin. Tradit. Herb. Drug, 43(2): 332-335.
    
11. Liang, H., Y. Liu, L. Han, J. He, W.Q. Zhang and A.G. Ma, 2011. Effects of Laurencia terpenoid extract on oxidative stress and expression of p53 and MMP-9 in H22 mice. Chin. Pharm. Bull., 27(8): 1183-1184.
    Direct Link
12. Liu, X.J., W.C. Wang, H.J. Wang, L.L. Zhang, L.Q. Liu, Y.J. Wang and C.B. Wang, 2009. A polypeptide from Chlamys farreri inhibits UVB-induced HaCaT cells apoptosis via the Apaf-1/caspase-9 and Smac/XIAP signaling pathway. Chin. J. Oceanol. Limn., 27(3): 587-593.
    Direct Link
13. Liu, Y., J. Wang, Z.S. Zhang and J. Wang, 2012. Study on antioxidative peptide preparation from scallop meat and in vitro antioxidant tests. Proceedings of the Academic Annual Conference of China Society of Fisheries. China Society of Fisheries, Zhengzhou, pp: 270.
    
14. Ngo, D.H., T.S. Vo, D.N. Ngo, I. Wijesekara and S.K. Kim, 2012. Biological activities and potential health bene?ts of bioactive peptides derived from marine organisms. Int. J. Biol. Macromol., 51(4): 378-383.
    CrossRef    PMid:22683669    Direct Link
15. Rameshkumar, G., S. Ravichandran, G. Kaliyavarathan and T.T.P. Ajithkumar, 2009. Antimicrobial peptide from the crab, Thalamita crenata (Latreille, 1829). World J. Fish Marine Sci., 1(2): 74-79.
    Direct Link
16. Sirak, I., J. Petera, J. Hatlova, M. Vosmik, B. Melichar, J. Dvorak, V. Tycova, Z. Zoul and M. Lesko, 2009. Expression of p53, p21 and p16 does not correlate with response to preoperative chemoradiation in gastric carcinoma. Hepato-Gastroenterol., 56(93): 1213-1218.
    Direct Link
17. Wang, W.L. and G. Meng, 2003. The relationship between caspase and survivin and their expressions and meanings in the hepatocellular carcinoma. Chin. J. Hepatob. Surg., 11(5): 392-395.
    
18. Wang, C.B., R.Y. Yao, Z.T. Liu, W.Z. Zhong, X.P. Liu and Y.J. Wang, 2002. Protective effect of polypeptide from Chlamys farreri on hairless mice damaged by ultraviolet A. Acta Pharmacol. Sin., 23(9): 813-818.
    PMid:12230950    
19. Wang, C.B., B.X. Ding, S.B. Guo, Y.Z. Wang, Y.T. Han and Y.J. Wang, 2003. Protective effect of polypeptide from Chlamys farreri on mitochondria in human dermal fibroblasts irradiated by ultraviolet B. Acta Pharm. Sin., 24(7): 692- 696.
    Direct Link
20. Yao, R.Y. and C.B. Wang, 2002. Protective effects of polypeptide from Chlamys farreri on HeLa cells damaged by Ultraviolet A. Acta Pharmacol. Sin., 23(11): 1018-1022.
    PMid:12421479    Direct Link
21. Zhang, F.Y., J.Z. Zhang, S.B. Zou, Z.F. Cheng, Y. Cai and Y.H. Yuan, 2011. The expressions and clinical significances of p21, p27, p53 and Rb. Chin. J. Clin. Exp. Pathol., 27(6): 586-589.
    
22. Zhiwu, H., C. Xiao, L. Chengjuan, W. Yuejun, S. Mi and W. Chunbo, 2006. Free radical scavenging abilities of polypeptide from Chlamys farreri. Chin. J. Oceanol. Limn., 24(3): 325-328.
    Direct Link

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