Abstract

In the study, Lactobacillus plantarum 18-2, Leuconostoc citreum 37 and Leuconostoc citreum 39, which were isolated from Inner Mongolia traditional dairy products, were used to detect their biofilm formation ability by the micro-titer plate biofilm assays. The results showed that the biofilm-forming ability of the three strains differed along with the alteration of their nutritional and environmental conditions. The optimal biofilm formation conditions of L. plantarum 18-2 were in pH5.6 MRS broth without any additives for 12 h at the temperature of 30°C Otherwise, L. citreum 37 was reported the best biofilm formation ability, when it’s cultivated in pH 5.4 for 48 h at 30°C, while the culture in pH5.5 for 48 h at 30°C is most conducive to the biofilm forming of L. citreum 39. In addition, when sodium chloride or sucrose was added to MRS broth, the biofilm forming was inhibited as the concentration increased. Thus it can be seen that, L. plantarum and L. citreum 37 and 39 were capable of forming biofilm and the biofilm formation was strongly modulated by culture conditions and medium, including low pH; low temperature and high osmolality.

Keywords:

Biofilm, conditions, lactic acid bacteria,

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