Abstract

The objective of the present study is to develop callus induction and plant regeneration; an experiment was conducted to investigate the effect of growth regulators on callus induction in rice mature embryo culture. In this experiment, callus was initiated from the mature seed scutellumof two Malaysian indica rice, MRQ74 and MR269. Callus was induced using different concentrations of 2, 4-D and BAP on MS medium under dark condition or under 16/8 h photoperiod conditions. For complete plant regeneration, the calli of both varieties were planted for shoot regeneration on MS media supplemented with different concentrations of BAP and NAA, for root regeneration on IBA. The results revealed that the maximum callus inductions in MRQ74 and MR269 were observed in the MS medium containing 3 mg/L 2, 4-D with 0.1 mg/L BAP under dark conditions. Furthermore, our protocol uses mature seeds as the explants in both rice varieties in terms of callus induction. Additionally, green plant production of plants was also observed in all of the media tested for shoot regeneration and MS media supplemented with 3 mg/L of BAP and 0.1 mg/L of NAA was found to be responsible for the recovery of more highly regenerated shoots. Effective root induction (100%) was achieved in a medium containing 1mg/LIBA. The described protocol can be useful in the establishment of an in vitro system for rice that can be a major technique for a genetic transformation system and crop improvement.

Keywords:

Callus induction, growth regulators, illumination, plant regeneration,

References

1. Al-Jubair, T., U. Salma, N. Haque, F. Akter, I.J. Mukti, A.K.M.F. Haque and M.R. Ali, 2008. Callus induction and regeneration of local rice (Oryza sativa L.) variety topa. Asian J. Plant Sci., 7(5): 514-517.
   CrossRef    Direct Link
2. Amarawathi, Y., R. Singh, A.K. Singh, V.P. Singh, T. Mohapatra, T.R. Sharma and N.K. Singh, 2008. Mapping of quantitative trait loci for basmati quality traits in rice (Oryza sativa L.). Mol. Breeding, 21(1): 49-65.
   CrossRef    Direct Link
3. Biswas, A. and A.B. Mandal, 2007. Plant regeneration in different genotypes of indica rice. Indian J. Biotechnol., 6(4): 532-540.
   
4. Chu, Q.R. and T.P. Croughan, 1990. Genetics of plant regeneration in immature panicle culture of rice. Crop Sci., 30(6): 1194-1197.
   CrossRef    Direct Link
5. Endress, R., 1994. Plant Cell Biotechnology. Springer-Verlag, Berlin, Heidelberg, pp: 353.
   CrossRef    Direct Link
6. FAO, 2007. FAOSTAT. Retrieved from: http://faostat.fao.org/site/567/default.aspx#ancor.
   Direct Link
7. Gueye, T. and K.N. Ndir, 2010. In vitro production of double haploid plants from two rice species (Oryza sativa L. and Oryza glaberrima Steudt.) for the rapid development of new breeding material. Sci. Res. Essays, 5(7): 709-713.
   Direct Link
8. Hoque, M.E., M.S. Ali and N.H. Karim, 2007. Embryogenic callus induction and regeneration of Elite Bangladeshi Indica rice cultivars. Plant Tissue Cult. Biotechnol., 17(1): 65-70.
   Direct Link
9. Htwe, N.N., M. Maziah, H.C. Ling, F.Q. Zaman and A.M. Zain, 2011. Responses of some selected Malaysian rice genotypes to callus induction under in vitro salt stress. Afr. J. Biotechnol., 10(3): 350-362.
   Direct Link
10. International Rice Genome Sequencing Project, 2005. The map-based sequence of the rice genome. Nature, 436: 793-800.
    CrossRef    PMid:16100779    Direct Link
11. Joyia, F.A. and M.S. Khan, 2012. Reproducible and expedient rice regeneration system using in vitro grown plants. Afr. J. Biotechnol., 11(1): 138-144.
    Direct Link
12. Kadhimi, A.A., A.N. Alhasnawi, A. Isahak, M.F. Ashraf, A. Mohamad, F. Doni, W. Mohtar, W.M.W. Yusoff, C. Radziah and C.R.C.M. Zain, 2014a. Effect of genotype and growth regulators in induction of embryogenic callus in rice. J. Pure Appl. Microbiol., 8(6): 4573-4578.
    Direct Link
13. Kadhimi, A.A., A.N. Alhasnawi, A. Mohamad, W.M.W. Yusoff and C.R.B.C.M. Zain, 2014b. Tissue culture and some of the factors affecting them and the micropropagation of strawberry. Life Sci. J., 11(8): 484-493.
    Direct Link
14. Khush, G.S., 2005. What it will take to feed 5.0 billion rice consumers in 2030. Plant Mol. Biol., 59(1): 1-6.
    CrossRef    PMid:16217597    Direct Link
15. Kunanuvatchaidach, R., I.D. Godwin and S.W. Adkins, 1995. High efficiency plant regeneration from callus induced on mature indica rice caryopses. Aust. J. Bot., 433(3): 337-348.
    CrossRef    
16. Li-na, Y., L. Xia and W. Dan, 2010. The comparison in tissue culture ability of mature embryo in different cultivars of rice. Agric. Sci. China, 9(6): 840-846.
    CrossRef    Direct Link
17. Mannan, M.A., T.C. Sarker, M.T. Akhter, A.H. Kabir and M.F. Alam, 2013. Indirect plant regeneration in aromatic rice (Oryza sativa L.) var. 'Kalijira' and 'Chinigura'. Acta Agr. Slov., 101(2): 231-238.
    CrossRef    Direct Link
18. Meneses, A., D. Flores, M. Mu-oz, G. Arrieta and A.M. Espinoza, 2005. Effect of 2,4-D, hydric stress and light on indica rice (Oryza sativa) somatic embryogenesis. Rev. Biol. Trop., 53(3-4): 361-368.
    CrossRef    PMid:17354447    Direct Link
19. MIATE (Malaysia International Agrotourism Exhibition), 2012. MARDI Padi MR 269. Retrieved from: https://blogmardi.wordpress.com/2012/12/03/mardi-hasilkan-varieti-padi-baru-penuhi-bekalan-beras-negara/#more-814.
    Direct Link
20. Mohd Zain, C.R.C., A.K. Siti Nurkhalida, Z. Zainal and I. Ismanizan, 2012. Effect of illumination casein hydrolysate and proline on callus induction of Oryza sativa L. cv. MR219. Malays. Appl. Biol., 41(1): 37-41.
    Direct Link
21. Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plantarum, 15(3): 473-497.
    CrossRef    Direct Link
22. Nabors, M.W., J.W. Heyser, T.A. Dykes and K.J. Demott, 1983. Long-duration, high-frequency plant regeneration from cereal tissue cultures. Planta, 157(5): 385-391.
    CrossRef    PMid:24264334    Direct Link
23. Pan, M.J. and J.V. Staden, 1998. The use of charcoal in in vitro culture – A review. Plant Growth Regul., 26(3): 155-163.
    CrossRef    Direct Link
24. Peterson, G. and R. Smith, 1991. Effect of abscisic acid and callus size on regeneration of American and international rice varieties. Plant Cell Rep., 10(1): 35-38.
    CrossRef    PMid:24226161    Direct Link
25. Ramli, A., O. Omar, S. Abdullah and A.R. dan Muhammad Harun, 2008. MRQ74 -A new aromatic rice variety. Bul. Teknol. Tanaman, Bil., 5: 15-20.
    
26. Rueb, S., M. Leneman, R.A. Schilperoort and L.A.M. Hensgens, 1994. Efficient plant regeneration through somatic embryogenesis from callus induced on mature rice embryos (Oryza sativa L.). Plant Cell, Tissue Organ Cult., 36(2): 259-264.
    CrossRef    Direct Link
27. Satyanarayana, A., 2005. System of rice intensification: An innovative method to produce more with less water and inputs. Proceeding of the 4th IWMI-Tata Annual Partners' Meeting. IRMA, Anand, India, February 24-26.
    
28. Seraj, Z.I., Z. Islam, M.O.T. Faruque and S. Ahmed, 1997. Indemnification of the regeneration potential of embryo derived calluses from various indica rice varieties. Plant Cell Tiss. Org., 48: 9-13.
    
29. Shanthi, P., S. Jebaraj, S. Geetha and N. Aananthi, 2010. Effect of growth regulators on callus induction in rice embryo culture. Electron. J. Plant Breeding., 1(4): 1200-1207.
    Direct Link
30. Silvarajan, L., R. Nulit and F.Q. Zaman, 2012. Effects of plant growth regulators on In vitro regeneration of Malaysian indica rice (Oryza sativa L.) cv. MR219 by shoot apical meristem. Asian J. Agr. Res., 6(4): 180-187.
    Direct Link
31. Sripichitt, P. and P. Cheewasestatham, 1994. Plant regeneration from embryo derived callus of aromatic rice (Oryza sativa L.) variety Khao Dawk Mali 105. Kasetsart J. Nat. Sci., 28: 27-37.
    
32. Suhaimee, S., M.R. Rabu, A.K. Ali and A. Ramli, 2009. Ex ante assessment on feasibility of Maswangi (MRQ 74) commercialization. Econ. Technol. Manage. Rev., 4: 49-56.
    Direct Link
33. Summart, J., S. Panichajakul, P. Prathepha and P. Thanonkeo, 2008. Callus induction and influence of culture condition and culture medium on growth of thai aromatic rice, khao Dawk Mali 105, cell culture. World Appl. Sci. J., 5(2): 246-251.
    Direct Link
34. Tariq, M., G. Ali, F. Hadi, S. Ahmad, N. Ali and A.A. Shah, 2008. Callus induction and in vitro plant regeneration of rice (Oryza sativa L.) under various conditions. Pak. J. Biol. Sci., 11(2): 255-259.
    CrossRef    PMid:18817199    Direct Link
35. Vaughan, D.A., B.R. Lu and N. Tomooka, 2008. The evolving story of rice evolution. Plant Sci., 174(4): 394-408.
    CrossRef    
36. Zhao, L., H. Zhou, L. Lu, L. Liu, X. Li, Y. Lin and S. Yu, 2009. Identification of quantitative trait loci controlling rice mature seed culturability using chromosomal segment substitution lines. Plant Cell Rep., 28(2): 247-256.
    CrossRef    PMid:19023575    Direct Link
37. Zinnah, K.M.A., N. Zobayer, S.U. Sikdar, L.N. Liza, M.A.N. Chowdhury and M. Ashrafuzzaman, 2013. In vitro regeneration and screening for salt tolerance in rice (Oryza sativa L.). Int. Res. J. Biol. Sci., 2(11): 29-36.
    Direct Link

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