Research Article | OPEN ACCESS
Rosemary Essential Oil and Lyophilized Extract as Natural Antioxidant Source to Prevent Lipid Oxidation in Pork Sausage
Mirelli Bianchin, Daiane Pereira, Amalia Soares dos Reis, Jacqueline de Florio Almeida, Leticia Dangui da Silva, Cristiane de Moura and Solange Teresinha Carpes
Department of Chemistry, Federal Technological University of Parana (UTFPR), P.O. Box 591,
85503-390, Pato Branco, PR, Brazil
Advance Journal of Food Science and Technology 2017 5:210-217
Received: May 8, 2017 | Accepted: June 23, 2017 | Published: July 25, 2017
Abstract
The aim of this study was to evaluate the effect of the use of Rosemary Essential Oil (REO) and Lyophilized Rosemary Extract (LRE) on the oxidative stability of pork sausages stored at -12°C. Potential antioxidants in sausages were determined by thiobarbituric acid reactive substances (TBARS) and expressed in mg of malonaldehyde MDA/kg. Measurements were taken one day each week starting from production day until 49 days after. The TBARS values increased over time, starting with a value of 0.45 and ending with 2.89 mg MDA/kg. The control treatment suffered more intense oxidation than those with Sodium Erythorbate (SE) and natural antioxidants. The lipid oxidation of sausage was significantly inhibited at 49 days of frozen storage in the presence of LRE when compared to the control (47.28%). The sausage containing LRE and REO showed a 75.67% and 56.41% acceptance rate, respectively. The LRE exhibited an excellent inhibitory effect on lipid oxidation of sausage and it has potential to use in meat products.
Keywords:
Antioxidant activity, lipid oxidation, meat products, Rosmarinus officinalis, TBARS,
References
-
Afonso, M.S., A.M.D.O. Silva, E.B. Carvalho, D.P. Rivelli, S.B. Barros, M.M. Rogero, A.M. Lottenberg, R.P. Torres and J. Mancini-Filho, 2013. Phenolic compounds from rosemary (Rosmarinus officinalis L.) attenuate oxidative stress and reduce blood cholesterol concentrations in diet-induced hypercholesterolemic rats. Nutr. Metab., 10(1): 19.
CrossRef PMid:23374457 PMCid:PMC3586571 Direct Link -
Ahn, M.R, S. Kumazawa, T. Hamasaka, K.S. Bang and T. Nakayama, 2004. Antioxidant activity and constituents of propolis collected in various areas of Korea. J. Agr. Food Chem., 52(24): 7286-7292.
CrossRef PMid:15563208 Direct Link -
Almeida, J.F., A.S. dos Reis, L.F.S. Heldt, D. Pereira, M. Bianchin, C. de Moura, M.V. Plata-Oviedo, C.W.I. Haminiuk, I.S. Ribeiro, C.F.P. da Luz and S.T. Carpes, 2017. Lyophilized bee pollen extract: A natural antioxidant source to prevent lipid oxidation in refrigerated sausages. LWT-Food Sci. Technol., 76(Part B): 299-305.
Direct Link -
Aouadi, D., G. Luciano, V. Vasta, S. Nasri, D.M.R. Brogna, S. Abidi, A. Priolo and H.B. Salem, 2014. The antioxidant status and oxidative stability of muscle from lambs receiving oral administration of Artemisia herba alba and Rosmarinus officinalis essential oils. Meat Sci., 97(2): 237-243.
CrossRef PMid:24583334 Direct Link -
Brand-Williams, W., M.E. Cuvelier and C. Berset, 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Technol., 28(1): 25-30.
CrossRef -
Brasil, 2000. Regulamentos tecnicos de identidade e qualidade de carne mecanicamente separada, de mortadela, de linguiça e de salsicha. Ministerio da Agricultura, Pecuaria e Abastecimento, Instruçao Normativa N4, de 31 de Março de 2000. Disponível, Brasília, DF, DOU.
-
Carpes, S.T., S.M. de Alencar, I.S.R. Cabral, T.L.C. Oldoni, G.B. Mourão, C.W.I. Haminiuk, C.F.P. da Luz and M.L. Masson, 2013. Polyphenols and palynological origin of bee pollen of Apis mellifera L. from Brazil. Characterization of polyphenols of bee pollen. CyTA - J. Food, 11(2): 150-161.
CrossRef Direct Link -
Casarotti, S.N. and N. Jorge, 2012. Antioxidant activity of rosemary extract in soybean oil under thermoxidation. J. Food Process. Pres., 38(1): 136-145.
CrossRef Direct Link -
Chen, X., Y. Zhang, Y. Zu, L. Yang, Q. Lu and W. Wang, 2014. Efeitos antioxidantes de extratos de alecrim em óleo de girassol em comparação com antioxidantes sintéticos. Int. J. Food Sci. Tech., 49: 385-391.
CrossRef -
Das, A.K., V. Rajkumar, A.K. Verma and D. Swarup, 2012. Moringa oleiferia leaves extract: A natural antioxidant for retarding lipid peroxidation in cooked goat meat patties. Int. J. Food Sci. Tech., 47(3): 585-591.
CrossRef Direct Link -
Dutcosky, S.D., 2007. Analise Sensorial de Alimentos. 2nd Edn., Champagnat, Curitiba, pp: 210.
-
Dvorackova, E., M. Snoblova and P. Hrdlicka, 2014. Content of phenolic compounds in herbs used in the Czech Republic. Int. Food Res. J., 21(4): 1495-1500.
Direct Link -
Erkan, N., G. Ayranci and E. Ayranci, 2008. Antioxidant activities of rosemary (Rosmarinus Officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol. Food Chem., 110(1): 76-82.
CrossRef PMid:26050168 Direct Link -
Falowo, A.B., P.O. Fayemi and V. Muchenje, 2014. Natural antioxidants against lipid–protein oxidative deterioration in meat and meat products: A review. Food Res. Int., 64: 171-181.
CrossRef Direct Link -
Hac-Szymanczuk, E., E. Lipinska and M. Stasiuk, 2011. The effect of rosemary preparations on the microbial quality and tbars value of model pork batters. Acta Sci. Pol. Technol. Aliment., 10(2): 165-174.
Direct Link -
Haminiuk, C.W.I., M.S.V. Plata-Oviedo, A.R. Guedes, A.P. Stafussa, E. Bona and S.T. Carpes, 2011. Chemical, antioxidant and antibacterial study of Brazilian fruits. Int. J. Food Sci. Tech., 46(7): 1529-1537.
CrossRef Direct Link -
Han, J. and K.S. Rhee, 2005. Antioxidant properties of selected Oriental non-culinary/nutraceutical herb extracts as evaluated in raw and cooked meat. Meat Sci., 70(1): 25-33.
CrossRef PMid:22063277 Direct Link -
Hernández-Hernández, E., E. Ponce-Alquicira, M.E. Jaramillo-Flores and I. Guerrero Legarreta, 2009. Antioxidant effect rosemary (Rosmarinus officinalis L.) and oregano (Origanum vulgare L.) extracts on TBARS and colour of model raw pork batters. Meat Sci., 81(2): 410-417.
CrossRef PMid:22064182 Direct Link -
Herrero, M., M. Plaza, A. Cifuentes and E. Ibá-ez, 2010. Green processes for the extraction of bioactives from rosemary: Chemical and functional characterization via ultra-performance liquid chromatography-tandem mass spectrometry and in-vitro assays. J. Chromatogr. A, 1217(16): 2512-2520.
CrossRef PMid:19945706 Direct Link -
Hossain, M.B., C. Barry-Ryan, A.B. Martin-Diana and N.P. Brunton, 2011. Optimisation of accelerated solvent extraction of antioxidant compounds from rosemary (Rosmarinus officinalis L.), marjoram (Origanum majorana L.) and oregano (Origanum vulgare L.) using response surface methodology. Food Chem., 126(1): 339-346.
CrossRef Direct Link -
Jongberg, S., M.A. Tørngren, A. Gunvig, L.H. Skibsted and M.N. Lund, 2013. Effect of green tea or rosemary extract on protein oxidation in Bologna type sausages prepared from oxidatively stressed pork. Meat Sci., 93(3): 538-546.
CrossRef PMid:23273462 Direct Link -
Keokamnerd, T., J.C. Acton, I.Y. Han and P.L. Dawson, 2008. Effect of commercial Rosemary oleoresin preparations on ground chicken thigh meat quality packaged in a high-oxygen atmosphere. Poultry Sci., 87(1): 170-179.
CrossRef PMid:18079467 Direct Link -
Kontogianni, V.G., G. Tomic, I. Nikolic, A.A. Nerantzaki, N. Sayyad, S. Stosic-Grujicic, I. Stojanovic, I.P. Gerothanassis and A.G. Tzakos, 2013. Phytochemical profile of Rosmarinus officinalis and Salvia officinalis extracts and correlation to their antioxidant and anti-proliferative activity. Food Chem., 136(1): 120-129.
CrossRef PMid:23017402 Direct Link -
Lapornik, B., M. Prošek and A.G. Wondra, 2005. Comparison of extracts prepared from plant by-products using different solvents and extraction time. J. Food Eng., 71(2): 214-222.
CrossRef Direct Link -
Lara, M.S., J.I. Gutierrez, M. Timón and A.I. Andrés, 2011. Evaluation of two natural extracts (Rosmarinus officinalis L. and Melissa officinalis L.) as antioxidants in cooked pork patties packed in MAP. Meat Sci., 88(3): 481-488.
CrossRef PMid:21345605 Direct Link -
Lianhe, Z., H. Xing, W. Li and C. Zhengxing, 2012. Physicochemical properties, chemical composition and antioxidant activity of Dalbergia odorifera T. Chen seed oil. J. Am. Oil Chem. Soc., 89(5): 883-890.
CrossRef Direct Link -
Maldini, M., P. Montoro, R. Addis, C. Toniolo, G.L. Petretto, M. Foddai, M. Nicoletti and G. Pintore, 2016. A new approach to discriminate Rosmarinus officinalis L. plants with antioxidant activity, based on HPTLC fingerprint and targeted phenolic analysis combined with PCA. Ind. Crop. Prod., 94: 665-672.
CrossRef Direct Link -
Mariutti, L.R.B. and N. Bragagnolo, 2007. Review: Natural antioxidants from the Lamiaceae family. Application in food products. Braz. J. Food Technol., 10(2): 96-103.
Direct Link -
Mishra, K., H. Ojha and N.K. Chaudhury, 2012. Estimation of antiradical properties of antioxidants using DPPH assay: A critical review and results. Food Chem., 130(4): 1036-1043.
CrossRef Direct Link -
Mulinacci, N., M. Innocenti, M. Bellumori, C. Giaccherini, V. Martini and M. Michelozzi, 2011. Storage method, drying processes and extraction procedures strongly affect the phenolic fraction of rosemary leaves: An HPLC/DAD/MS study. Talanta, 85(1): 167-176.
CrossRef PMid:21645686 Direct Link -
Napoli, E.M., G. Curcuruto and G. Ruberto, 2010. Screening of the essential oil composition of wild Sicilian Rosemary. Biochem. Syst. Ecol., 38(4): 659-670.
CrossRef Direct Link -
Naveena, B.M., S. Vaithiyanathan, M. Muthukumar, A.R. Sen, Y.P. Kumar, M. Kiran, V.A. Shaju and K.R. Chandran, 2013. Relationship between the solubility, dosage and antioxidant capacity of carnosic acid in raw and cooked ground buffalo meat patties and chicken patties. Meat Sci., 95(2): 195-202.
CrossRef PMid:23743029 Direct Link -
Okpala, C.O.R., 2016. Lipid autoxidation in ozone-processed crustacea under cold storage: A treatise. Lipid Technol., 28(5-6): 93-95.
CrossRef Direct Link -
Olmedo, R.H., C.M. Asensio and N.R. Grosso, 2015. Thermal stability and antioxidant activity of essential oils from aromatic plants farmed in Argentina. Ind. Crop. Prod., 69: 21-28.
CrossRef Direct Link -
Osawa, C.C., P.E. de Felício and L.A.G. Gonçalves, 2005. TBA test applied to meats and their products: Traditional, modified and alternative methods. Quim. Nova, 28(4): 655-663.
CrossRef Direct Link -
Pateiro, M., J.M. Lorenzo, I.R. Amado and D. Franco, 2014. Effect of addition of green tea, chestnut and grape extract on the shelf-life of pig liver pate. Food Chem., 147: 386-394.
CrossRef PMid:24206734 Direct Link -
Pérez-Fons, L., M.T. Garzón and V. Micol, 2010. Relationship between the antioxidant capacity and effect of rosemary (Rosmarinus officinalis L.) polyphenols on membrane phospholipid order. J. Agr. Food Chem., 58(1): 161-171.
CrossRef PMid:19924866 Direct Link -
Pulido, R., L. Bravo and F. Saura-Calixto, 2000. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. J. Agr. Food Chem., 48: 3396-3402.
CrossRef PMid:10956123 -
Raharjo, S., J.N. Sofos and G.R. Schmidt, 1992. Improved speed, specificity, and limit of determination of an aqueous acid extraction thiobarbituric acid-C18 method for measuring lipid peroxidation in beef. J. Agr. Food Chem., 40(11): 2182-2185.
CrossRef Direct Link -
Raškovic, A., I. Milanovic, N. Pavlovic, T. Cebovic, S. Vukmirovic and M. Mikov, 2014. Antioxidant activity of rosemary (Rosmarinus officinalis L.) essential oil and its hepatoprotective potential. BMC Complem. Altern. M., 14: 225.
CrossRef PMid:25002023 PMCid:PMC4227022 Direct Link -
Re, R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang and C. Rice-Evans, 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio. Med., 26(9-10): 1231-1237.
CrossRef -
Realini, C.E. and B. Marcos, 2014. Active and intelligent packaging systems for a modern society. Meat Sci., 98(3): 404-419.
CrossRef PMid:25034453 Direct Link -
Rocha, F.D., A.R. Soares, P.J. Houghton, R.C. Pereira, M.A. Kaplan and V.L. Teixeira, 2007. Potential cytotoxic activity of some Brazilian seaweeds on human melanoma cells. Phytother. Res., 21(2): 170-175.
CrossRef PMid:17133653 Direct Link -
Savadkoohi, S., H. Hoogenkamp, K. Shamsi and A. Farahnaky, 2014. Color, sensory and textural attributes of beef frankfurter, beef ham and meat-free sausage containing tomato pomace. Meat Sci., 97(4): 410-418.
CrossRef PMid:24769097 Direct Link -
Sáyago-Ayerdi, S.G., A. Brenes, A. Viveros and I. Go-i, 2009. Antioxidative effect of dietary grape pomace concentrate on lipid oxidation of chilled and long-term frozen stored chicken patties. Meat Sci., 83(3): 528-533.
CrossRef PMid:20416664 Direct Link -
Sebranek, J.G., V.J.H. Sewalt, K.L. Robbins and T.A. Houser, 2005. Comparison of a natural rosemary extract and BHA/BHT for relative antioxidant effectiveness in pork sausage. Meat Sci., 69(2): 289-296.
CrossRef PMid:22062821 Direct Link -
Seneviratne, K.N., W.C. Prasadani and B. Jayawardena, 2016. Phenolic extracts of coconut oil cake: A potential alternative for synthetic antioxidants. Food Sci. Technol., 36(4): 591-597.
CrossRef Direct Link -
Shah, M.A., S.J.D. Bosco and S.A. Mir, 2014. Plant extracts as natural antioxidants in meat and meat products. Meat Sci., 98(1): 21-33.
CrossRef PMid:24824531 Direct Link -
Singleton, V.L., R. Orthofer and R.M. Lamuela-Raventós, 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Method. Enzymol., 299: 152-178.
CrossRef Direct Link -
Sun-Waterhouse, D., J. Zhou, G.M. Miskelly, R. Wibisono and S.S. Wadhwa, 2011. Stability of encapsulated olive oil in the presence of caffeic acid. Food Chem., 126(3): 1049-1056.
CrossRef Direct Link -
Švarc-Gajic, J., Z. Stojanovic, A.S. Carretero, D.A. Román, I. Borrás and I. Vasiljevic, 2013. Development of a microwave-assisted extraction for the analysis of phenolic compounds from Rosmarinus officinalis. J. Food Eng., 119(3): 525-532.
CrossRef Direct Link -
Teruel, M.R., M.D. Garrido, M.C. Espinosa and M.B. Linares, 2015. Effect of different format-solvent rosemary extracts (Rosmarinus officinalis) on frozen chicken nuggets quality. Food Chem., 172: 40-46.
CrossRef PMid:25442521 Direct Link -
Vallverdú-Queralt, A., J. Regueiro, M. Martínez-Huélamo, J.F.R. Alvarenga, L.N. Leal and R.M. Lamuela-Raventos, 2014. A comprehensive study on the phenolic profile of widely used culinary herbs and spices: Rosemary, thyme, oregano, cinnamon, cumin and bay. Food Chem., 154: 299-307.
CrossRef PMid:24518346 Direct Link -
Venturini, A.C., A.D. Cavenaghi, C.J.C. Castillo and E.M. Qui-ones, 2011. Sensory and microbiological evaluation of uncured fresh chicken sausage with reduced fat content. Ciênc. Tecnol. Aliment., 31(3): 629-634.
CrossRef Direct Link -
Vicente, G., S. Molina, M. González-Vallinas, M.R. García-Risco, T. Fornari, G. Reglero and A.R. de Molina, 2013. Supercritical rosemary extracts, their antioxidant activity and effect on hepatic tumor progression. J. Supercrit. Fluid., 79: 101-108.
CrossRef Direct Link -
Windisch, W., K. Schedle, C. Plitzner and A. Kroismayr, 2008. Use of phytogenic products as feed additives for swine and poultry. J. Anim. Sci., 86(14 Suppl): E140-E148.
CrossRef PMid:18073277 Direct Link -
Yosr, Z., C. Hnia, T. Rim and B. Mohamed, 2013. Changes in essential oil composition and phenolic fraction in Rosmarinus officinalis L. var. typicus Batt. organs during growth and incidence on the antioxidant activity. Ind. Crops Prod., 43: 412-419.
CrossRef Direct Link -
Zaouali, Y., T. Bouzaine and M. Boussaid, 2010. Essential oils composition in two Rosmarinus officinalis L. varieties and incidence for antimicrobial and antioxidant activities. Food Chem. Toxicol., 48(11): 3144-3152.
CrossRef PMid:20728499 Direct Link
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The authors have no competing interests.
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