Abstract

The quantitative effects of extraction temperature, extraction time and ratio of solid to liquid on yield of Bletilla striata polysaccharides were investigated using Response Surface Methodology (RSM). The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed by appropriate statistical methods. By solving the regression equation and also by analyzing the response surface contour plots, the optimal polysaccharides extraction conditions were determined: Extraction temperature of 92.8ºC, extraction time of 2.33 h and liquid to solid ratio 67.2 mL/g. Under these conditions, maximum yield of polysaccharides 31.41% can be achieved.

Keywords:

Bletilla striata, extraction, polysaccharide, response surface methodology,

References

1. Diao, H., X. Li, J. Chen, Y. Luo, X. Chen, L. Dong, C. Wang, C. Zhang and J. Zhang, 2008. Bletilla striata polysaccharide stimulates inducible nitric oxide synthase and proinflammatory cytokine expression in macrophages. J. Biosci. Bioeng., 105(2): 85-89.
   CrossRef    PMid:18343332    Direct Link
2. Dong, L., S. Xia, Y. Luo, H. Diao, J. Zhang, J. Chen and J. Zhang, 2009. Targeting delivery oligonucleotide into macrophages by cationic polysaccharide from Bletilla striata successfully inhibited the expression of TNF-alpha. J. Control Release, 134(3): 214-220.
   CrossRef    PMid:19073226    Direct Link
3. Dubois, M., K.A. Gilles, J.K. Hamilton, P.A. Rebers and F. Smith, 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem., 28(3): 350-356.
   CrossRef    
4. Hirano, T., T. Godo, M. Mii and K. Ishikawa, 2005. Cryopreservation of immature seeds of Bletilla striata by vitrification. Plant Cell Rep., 23(8): 534-539.
   CrossRef    PMid:15565321    Direct Link
5. Morita, H., K. Koyama, Y. Sugimoto and J. Kobayashi, 2005. Antimitotic activity and reversal of breast cancer resistance protein-mediated drug resistance by stilbenoids from Bletilla striata. Bioorg. Med. Chem. Lett., 15(4): 1051-1054.
   CrossRef    PMid:15686910    Direct Link
6. RenJie, L., 2008. Optimization of extraction process of Glycyrrhiza glabra polysaccharides by response surface methodology. Carbohyd. Polym., 74(4): 858-861.
   CrossRef    Direct Link
7. Wang, C., J. Sun, Y. Luo, W. Xue, H. Diao, L. Dong, J. Chen and J. Zhang, 2006. A polysaccharide isolated from the medicinal herb Bletilla striata induces endothelial cells proliferation and vascular endothelial growth factor expression in vitro. Biotechnol. Lett., 28(8): 539-543.
   CrossRef    PMid:16614890    Direct Link
8. Wu, Y., S.W. Cui, J. Tang and X.H. Gu, 2007. Optimization of extraction process of crude polysaccharides from boat-fruited sterculia seeds by response surface methodology. Food Chem., 105(4): 1599-1605.
   CrossRef    Direct Link
9. XuJie, H. and C. Wei, 2008. Optimization of extraction process of crude polysaccharides from wild edible BaChu mushroom by response surface methodology. Carbohyd. Polym., 72(1): 67-74.
   CrossRef    Direct Link
10. Ye, C.L. and C.J. Jiang, 2011. Optimization of extraction process of crude polysaccharides from Plantago asiatica L. by response surface methodology. Carbohyd. Polym., 84(1): 495-502.
    CrossRef    Direct Link
11. Yin, G.H. and Y.L. Dang, 2008. Optimization of extraction technology of the Lycium barbarum polysaccharides by Box-Behnken statistical design. Carbohyd. Polym., 74(3): 603-610.
    CrossRef    Direct Link
12. Zhang, G.W., L. He and M.M. Hu, 2011. Optimized ultrasonic-assisted extraction of flavonoids from Prunella vulgaris L. and evaluation of antioxidant activities in vitro. Innov. Food Sci. Emerg., 12(1): 18-25.
    CrossRef    Direct Link
13. Zhong, K. and Q. Wang, 2010. Optimization of ultrasonic extraction of polysaccharides from dried longan pulp using response surface methodology. Carbohyd. Polym., 80(1): 19-25.
    CrossRef    Direct Link

Competing interests

The authors have no competing interests.

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