Research Article | OPEN ACCESS
Influence of Different Solvents in the Recovery and Pre-Purification of Pectinases from Aspergillus niger
1Marcia M. Santin Trentini, 1Ana Paula Rossetto, 1Ilizandra Aparecida Fernandes, 1Carolina Elisa Demaman Oro, 1Rogerio Luis Cansian, 1, 2Marco Di Luccio and 1Eunice Valduga
1Department of Food Engineering, URI Erechim, Av. Sete de Setembro, 1621-Erechim-RS,
99700-000, Brazil
2Department of Chemical and Food Engineering, Federal University of Santa Catarina, Caixa Postal 476, Trindade, Florian
Advance Journal of Food Science and Technology 2018 3:69-76
Received: August 10, 2017 | Accepted: October 10, 2017 | Published: April 25, 2018
Abstract
The present work aimed at studying the influence of different solvents (n-propyl alcohol, isopropyl alcohol and acetone) in the recovery and pre-purification of pectinases (exo-polygalacturonase-exo-PG, pectinmethylesterase-PME and pectin lyase-PMGL) from Aspergillus niger. The best results were obtained using isopropyl alcohol in the precipitated phase with purification factor and recovery of 2.1-fold and 20.5% for exo-PG, 7.9-fold and 77.8% for PME and 2.5-fold and 216% for PMGL enzyme. The studied method is promising in the recovery and pre-purification of pectinases enzymes.
Keywords:
Pectin lyase, pectinmethylesterase, polygalacturonase, precipitation, purification, solvents,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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