Research Article | OPEN ACCESS
Bactericidal Effect of Ultrasound on the Microbiota of Raw Milk Cream
1Pino Alexandra, 1Caballero Luz A. and 1, 2Rivera Maria E.
1University of Pamplona. Master's degree in Food Science and Technology. Group Research GIBA, University Campus, The Buque, Pamplona, Norte of Santander, Colombia
2University of Pamplona. Master's degree in Food Science and Technology. Environmental Research Group Water, Air and Soil GIAAS, University Campus, The Buque, Pamplona, Norte of Santander, Colombia
Advance Journal of Food Science and Technology 2018 SPL:179-183
Received: September 14, 2017 | Accepted: December 9, 2017 | Published: July 10, 2018
Abstract
The objective of this research was to evaluate the effect of Ultrasound on the microbiota present in raw milk cream in order to decrease the initial microbial load. Ultrasound offers an alternative in the conservation and preservation of food. The raw milk cream as a by-product of industrial processing is susceptible to deterioration by high microbial load and the presence of enzymes, which develop unwanted defects. The analysis did to themselves for triplicate, taking randomly a quantity average of cream, packed, labeled in Polyethylene bags low thickness (300 g) and refrigerated (4±2°C). The samples were evaluated microbiologically (mesophyll’s aerobic, total coliforms, fungi and yeasts), before and after treatment with ultrasound at a frequency of 37 KHz, with times of 2, 5 and 10 min, with temperatures of 30 and 40±2°C and stored in refrigeration (4±2°C) for 10 days (days 0, 3, 5, 7 and 10). The experimental data evaluated using a factorial design (time and temperature of exposure to US at 4 and 2 levels with three replicates for each treatment. An Analysis of Variance (ANOVA) was performed at a significance level of p≤0.05 and test POST HOC of Tukey using the statistical package SPSS v. 19.0 was shown that the treatment at 37 Hz for 10 min at a temperature of 40°C decreased the initial microbial load by 79%.
Keywords:
Frequency, fungi, mesophylls aerobes, temperature, total coliforms, yeasts,
References
-
Ahmed Ansari, J., M. Ismail and M. Farid, 2017. Investigation of the use of ultrasonication followed by heat for spore inactivation. Food Bioprod. Process, 104: 32-39.
CrossRef
-
AOAC, 2002a. Bacterial and Coliform Counts in Milk. Dry Rehydratable Film Methods. Sec. 17. 7. 03. Method 986.33, In: Official Methods of Analysis of AOAC International, AOAC International, Gaithersburg, MD.
-
AOAC, 2002b. Coliform and Escherichia coli Counts in Foods: Dry Rehydratable Film Methods. Sec. 17.3.04, Method 991.14. In: Cunniff, P.A. (Ed.), Official Methods of Analysis of AOAC International, 16th Edn., AOAC International, Gaithersburg, MD, pp: 13-15.
-
AOAC, 2002c. Yeast and Mold Counts in Foods. Sec. 17.2.09, Method 997.02. In: Official Methods of Analysis of AOAC International. AOAC International, Gaithersburg, MD.
-
Bermúdez-Aguirre, D., R. Mawson and G.V. Barbosa-Cánovas, 2008. Microstructure of fat globules in whole milk after thermosonication treatment. J. Food. Sci., 73(7): E325-E332.
CrossRef PMid:18803706
-
Chandrapala, J., C. Oliver, S. Kentish and M. Ashokkumar, 2012. Ultrasonics in food processing-food quality assurance and food safety. Trends Food Sci. Technol., 26(2): 88-98.
CrossRef
-
Farid, C., H. Zill and K.K. Muhammed, 2011. Applications of ultrasound in food technology: Processing, preservation and extraction. Ultrason. Sonochem., 18(4): 813-835.
CrossRef PMid:21216174
-
Chemat, F., N. Rombaut, A. Meullemiestre, M. Turk, S. Perino, A.S. Fabiano-Tixier and M. Abert-Vian, 2017. Review of green food processing techniques. Preservation, transformation, and extraction. Innov. Food Sci. Emerg. Technol., 41: 357-377.
CrossRef
-
Guerrero, S. and S. Alzamora, 2004. The paper of ultrasonic in foods preservation. Latin Am. Food., 244: 18-21.
-
Knoerzer, K., R. Buckow, F.J. Trujillo and P. Juliano, 2015. Multiphysics simulation of innovative food processing technologies. Food Eng. Rev., 7(2): 64-81.
CrossRef
-
Mason, T., F. Chemat and M. Ashokkumar, 2015. Power ultrasonic for food processing. Appl. High-Intensity Ultras., 27: 815-843.
-
Misra, N.N., M. Koubaa, S. Roohinejad, P. Juliano, H. Alpas, R.S. Inacio, J.A. Saraiva and F.J. Barba, 2017. Landmarks in the historical development of twenty first century food processing technologies. Food Res. Int., 97: 318-339.
CrossRef PMid:28578057
-
NTC 399, 2002. Raw Milk. 4th Edn., National Institute of Technical Standards and Certification. ICONTEC.
-
Schössler, K., H. Jäger, C. Büchner, S. Struck and D. Knorr, 2014. Non-Thermal Processing Ultrasonication. 2nd Edn., Reference Module in Food Science: From Encyclopedia of Food Microbiology, pp: 985-989.
PMid:25498935
-
Wu, T., X. Yu, A. Hu, L. Zhang, Y. Jin and M. Abid, 2015. Ultrasonic disruption of yeast cells: Underlying mechanism and effects of processing parameters. Innov. Food Sci. Emerg., 28: 59-65.
CrossRef
-
Zhao, B., O. Basir and G. Mittal, 2007. Innovation in food engineering: New techniques and products: Maximum components integration for image processing: An application of ultrasound for detection of small objects in containers. J. Food Pro. Eng., 30: 393-405.
CrossRef
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
|
|
|
ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
|
Information |
|
|
|
Sales & Services |
|
|
|