Research Article | OPEN ACCESS
Effects of Operation Conditions on Antioxidant and Iron Chelating Activity of Chemical Hydrolysates from Red Tilapia (Oreochromis sp.) Scales
Leidy Maritza Sierra and Jose Edgar Zapata
Universidad de Antioquia, Medellin, Colombia
Advance Journal of Food Science and Technology 2018 SPL:210-217
Received: September 14, 2017 | Accepted: December 26, 2017 | Published: July 10, 2018
Abstract
The aim of this study was to optimize the alkaline hydrolysis to extract protein from RTS with antioxidant and iron chelating activity. Red Tilapia (Oreochromis sp.) scales (RTS) have essential protein contents; therefore, those represent an opportunity to obtain hydrolysates, which may present biological activities of interest. For the extraction of the protein, it is possible to use chemical methods such as alkaline hydrolysis. The factors of central composite design (DOE) were NaOH concentration (0.5 to 2M), temperature (40 to 60°C) and percentage of scales (2.5 to 7.5%). The response variables were: protein (g/L), antioxidant capacity (ABTS and FRAP) and percentage of iron chelating activity (ICH). The hydrolysis time was evaluated in 2, 4 and 8 h in a 500 mL reactor with constant stirring. It was possible to obtain significant models for the variables assessed and those showed that with high levels of temperature, NaOH concentration and substrate concentration get a higher concentration of soluble protein without affecting the biological activities evaluated. The quantity of the protein obtained for the optimal DOE conditions was 20 g/L, with an extraction yield of 98%. The best time to get proteins with bioactivity was 2 h. This study evidenced the obtainment of protein hydrolysates from RTS with antioxidant and iron chelating activity using alkaline hydrolysis.
Keywords:
Alkaline, bioactive, extraction, hydrolysis, protein,
References
-
Anal, A.K., A. Noomhorm and P. Vongsawasdi, 2013. Protein Hydrolysates and Bioactive Peptides from Seafood and Crustacean Waste: Their Extraction, Bioactive Properties and Industrial Perspectives. In: Marine Proteins and Peptides: Biological Activities and Applications. John Wiley & Sons, New York, pp: 709-735.
CrossRef -
Batista, I., 1999. Recovery of proteins from fish waste products by alkaline extraction. Eur. Food Res. Technol., 210(2): 84-89.
CrossRef -
Benjakul, S., S. Senphan, T. Halldorsdottir and H. Kristinsson, 2014. Fish Protein Hydrolysates: Production, Bioactivities and Applications. Antioxidants and Functional Components in Aquatic Foods. 1st Edn., Reykjavik, Matil Ltd., Iceland, pp: 237-83.
-
Chalamaiah, M., B. Dinesh Kumar, R. Hemalatha and T. Jyothirmayi, 2012. Fish protein hydrolysates: Proximate composition, amino acid composition, antioxidant activities and applications: A review. Food Chem., 135(4): 3020-3038.
CrossRef PMid:22980905 -
Charoenphun, N., B. Cheirsilp, N. Sirinupong and W. Youravong, 2012. Calcium-binding peptides derived from tilapia (Oreochromis niloticus) protein hydrolysate. Eur. Food Res. Technol., 236(1): 57-63.
CrossRef -
Chen, D., X. Mu, H. Huang, R. Nie, Z. Liu and M. Zeng, 2014. Isolation of a calcium-binding peptide from tilapia scale protein hydrolysate and its calcium bioavailability in rats. J. Funct. Food., 6: 575-584.
CrossRef -
Choonpicharn, S., S. Jaturasitha, N. Rakariyatham, N. Suree and H. Niamsup, 2015. Antioxidant and antihypertensive activity of gelatin hydrolysate from Nile tilapia skin. J. Food Sci. Tech., 52(5): 3134-3139.
CrossRef PMid:25892821 PMCid:PMC4397345 -
Chuaychan, S. and S. Benjakul, 2016. Effect of maltodextrin on characteristics and antioxidative activity of spray-dried powder of gelatin and gelatin hydrolysate from scales of spotted golden goatfish. J. Food Sci. Tech., 53(9): 3583-3592.
CrossRef PMid:27777465 PMCid:PMC5069263 -
Chuaychan, S., S. Benjakul and H. Kishimura, 2017. Characteristics and gelling property of gelatin from scale of spotted golden goatfish (Parupeneus heptacanthus). J. Food Process. Pres., 41(5).
CrossRef -
Essuman, E.K., G.M. Ankar-Brewoo, I.W. Ofosu and J. Barimah, 2014. Optimization of protein extraction from fern frond using response surface methodology (rsm). Ife J. Sci., 16(1): 149-159.
-
Farvin, K.H.S., L.L. Andersen, H.H. Nielsen, C. Jacobsen, G. Jakobsen, I. Johansson and F. Jessen, 2014. Antioxidant activity of cod (Gadus morhua) protein hydrolysates: In vitro assays and evaluation in 5% fish oil-in-water emulsion. Food Chem., 149: 326-334.
CrossRef PMid:24295714 -
Foh, M.B.K., X. Wenshui, I. Amadou and Q. Jiang, 2011. Influence of pH shift on functional properties of protein isolated of tilapia (Oreochromis niloticus) muscles and of soy protein isolate. Food Bioprocess Tech., 5(6): 2192-2200.
CrossRef -
Harnedy, P.A. and R.J. FitzGerald, 2012. Bioactive peptides from marine processing waste and shellfish: A review. J. Funct. Food., 4(1): 6-24.
CrossRef -
He, S., C. Franco and W. Zhang, 2013. Functions, applications and production of protein hydrolysates from fish processing co-products (FPCP). Food Res. Int., 50(1): 289-297.
CrossRef -
Ingadottir, B. and H.G. Kristinsson, 2010. Gelation of protein isolates extracted from tilapia light muscle by pH shift processing. Food Chem., 118(3): 789-798.
CrossRef -
Je, J.Y., Z.J. Qian, H.G. Byun and S.K. Kim, 2007. Purification and characterization of an antioxidant peptide obtained from tuna backbone protein by enzymatic hydrolysis. Process Biochem., 42(5): 840-846.
CrossRef -
Latimer, G.W., 2012. Official Methods of analysis of AOAC International. 19th Edn., (No. 543/L357). AOAC International, Gaithersburg, Md.
-
Martínez-Alvarez, O., S. Chamorro and A. Brenes, 2015. Protein hydrolysates from animal processing by-products as a source of bioactive molecules with interest in animal feeding: A review. Food Res. Int., 73: 204-212.
CrossRef -
Mosquera, M., B. Gimenez, I.M. Da Silva, J.F. Boelter, P. Montero, M.C. Gomez-Guillen and A. Brandelli, 2014. Nanoencapsulation of an active peptidic fraction from sea bream scales collagen. Food Chem., 156: 144-150.
CrossRef PMid:24629950 -
Nolsøe, H. and I. Undeland, 2008. The acid and alkaline solubilization process for the isolation of muscle proteins: State of the Art. Food Bioprocess Tech., 2(1): 1-27.
CrossRef -
Nurdiyana, H., M.K. Siti Mazlina and M. Siti nor Fadhilah, 2008. Optimization of protein extraction from freeze dried fish waste using response surface methodology (RSM). Int. J. Eng. Technol., 5(1): 48-56.
-
Piva, G., M. Moschini, L. Fiorentini and F. Masoero, 2001. Effect of temperature, pressure and alkaline treatments on meat meal quality. Anim. Feed Sci. Tech., 89(1-2): 59-68.
CrossRef -
Raghavan, S. and H.G. Kristinsson, 2008. Antioxidative efficacy of alkali-treated tilapia protein hydrolysates: A comparative study of five enzymes. J. Agr. Food Chem., 56(4): 1434-1441.
CrossRef PMid:18247531 -
Raghavan, S. and H.G. Kristinsson, 2009. ACE-inhibitory activity of tilapia protein hydrolysates. Food Chem., 117(4): 582-588.
CrossRef -
Rawdkuen, S., S. Sai-Ut, S. Khamsorn, M. Chaijan and S. Benjakul, 2009. Biochemical and gelling properties of tilapia surimi and protein recovered using an acid-alkaline process. Food Chem., 112(1): 112-119.
CrossRef -
Sankar, S., S. Sekar, R. Mohan, S. Rani, J. Sundaraseelan and T.P. Sastry, 2008. Preparation and partial characterization of collagen sheet from fish (Lates calcarifer) scales. Int. J. Biol. Macromol., 42(1): 6-9.
CrossRef PMid:17936895 -
Sanmart’in, E., J.C. Arboleya, M. Villamiel and F. Javier Moreno, 2009. Recent advances in the recovery and improvement of functional proteins from fish processing by-products: Use of protein glycation as an alternative method. Compr. Rev. Food Sci. F., 8(4): 332-344.
CrossRef -
Silva, C.M.L.D., E. Spinelli and S.V. Rodrigues, 2015. Fast and sensitive collagen quantification by alkaline hydrolysis/hydroxyproline assay. Food Chem., 173: 619-623.
CrossRef PMid:25466067 -
Silva, J.F.X., K. Ribeiro, J.F. Silva, T.B. Cahú and R.S. Bezerra, 2014. Utilization of tilapia processing waste for the production of fish protein hydrolysate. Anim. Feed Sci. Tech., 196: 96-106.
CrossRef -
Undeland, I., S.D. Kelleher and H.O. Hultin, 2002. Recovery of functional proteins from herring (Clupea harengus) light muscle by an acid or alkaline solubilization process. J. Agr. Food Chem., 50(25): 7371-7379.
CrossRef PMid:12452661 -
Wangtueai, S. and A. Noomhorm, 2009. Processing optimization and characterization of gelatin from lizardfish (Saurida spp.) scales. LWT-Food Sci. Technol., 42(4): 825-834.
-
Wangtueai, S., S. Siebenhandl-Ehn and D. Haltrich, 2016. Optimization of the preparation of gelatin hydrolysates with antioxidative activity from lizardfish (Saurida spp.) scales gelatin. Chiang Mai J. Sci., 43(1): 1122-1133.
-
Zhou, P. and J.M. Regenstein, 2005. Effects of alkaline and acid pretreatments on alaska pollock skin gelatin extraction. J. Food Sci., 70(6): c392-c396.
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The authors have no competing interests.
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