Abstract

To optimize parameters influencing transformation efficiency is one of the first steps to develop molecular tools. Acinetobacter calcoaceticus is an emerging, potential application bacterium, even a cultivable symbiont that is poorly characterized due to a paucity of genetic tools and methods. Here we reported parameters allowing high efficiency transformation of A. calcoaceticus. Using a Orthogonal experimental design we found that growth phase, resistance and the amount of plasmid DNA significantly contribute to transformation efficiency. The highest efficiency (7.51×10³ transformants/μg DNA) was obtained at the growth phase of the bacterium (OD₆₀₀ = 0.5) using 700 ng of plasmid DNA under 200 Ohms resistance condition. The optimized electroporation parameters might provide a useful tool for genetic manipulation of A. calcoaceticus.

Keywords:

Evaluation, orthogonal experimental design, parameters, transformation,

References

Competing interests

The authors have no competing interests.

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Copyright

The authors have no competing interests.