Abstract

The aim of this study was to enhance the Coenzyme Q₁₀ (CoQ₁₀) production from Rhodobactersphaeroides. CoQ₁₀acts as an electron carrier and plays an important role in the aerobic respiration for the production of ATP. CoQ₁₀ has been proven to be effective for treatment of human diseases and widely used in food and pharmaceutical industry. In the present study, the ubiE gene located in CoQ₁₀ biosynthesis pathway was overexpressed in Rb. sphaeroides under strong pufoperon promoter and micro-aerobic growth conditions to enhance CoQ₁₀ production. The growth curve implied that effect of overexpression of ubiE on the growth of host cells was slight. The crude CoQ₁₀ production was enhanced by 80.61%, which was increased much higher than reported literature. The ubiE mRNA level was significantly increased compared to the wild type harboring empty vector as measured by quantitative RT-PCR. Furthermore, the crude CoQ₁₀ possessed strong hydroxyl radical scavenging activity as measured in vivo by zone of inhibition assay. The in vitro assay indicated that the crude CoQ₁₀ exhibited much stronger hydroxyl radical scavenging activity than normally used antioxidant vitamin C.

Keywords:

Anti-oxidation, CoQ₁₀, genetic engineering, hydroxyl radical Rb. sphaeroides, scavenging activity, ubiE,

References

Competing interests

The authors have no competing interests.

Open Access Policy

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Copyright

The authors have no competing interests.