Research Article | OPEN ACCESS
New Role of Rosea1 in Regulating Anthocyanin Biosynthetic Pathway in Hairy Root of Snapdragon (Antirrhinum majus L.)
1, 2An Zhang and 2Min-Long Cui
1Graduate School, Chinese Academy of Sciences, Beijing 100039, China
2Group of Molecular Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning 110016, China
Advance Journal of Food Science and Technology 2013 9:1174-1179
Received: May 17, 2013 | Accepted: June 10, 2013 | Published: September 05, 2013
Abstract
We investigated the transcriptional regulation of anthocyanin biosynthesis in hairy roots system by ectopically expressing Rosea1 and Delila and we found something different from previous research. The RT-PCR results revealed that Rosea1 could activate early and late biosynthetic genes tested, including CHS, DFR and ANS. Delila enhanced the expression of CHS weakly, but did not influence DFR or ANS. The two regulators, Rosea1 and Delila, failed to interplay each other. It was speculated that Delila would be ineffective in the absence of Rosea1, another MYB factor specifically controlling CHS may exist. This investigation provided a new way to increase anthocyanin content by over expressing a MYB factor, potentially to be used in the field of agriculture and food.
Keywords:
Anthocyanin biosynthesis, Antirrhinum majus, hairy root, snapdragon,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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