Research Article | OPEN ACCESS
Preparation and Enzyme-Linked Immunosorbent Assay of Monoclonal Antibody Against Ampicillin
1Yan Lu, 2Xuping Zhou, 3Hongyu Zhao, 4Jian Sun and 1Guojuan Wu
1College of Animal Science and Technology, Beijing University of Agriculture,
Beijing 102206, P.R. China
2College of Urban and Rural Development, Beijing University of Agriculture, Beijing 102206, P.R. China
3National Institute of Biological Sciences, Beijing 100101, P.R. China
4Department of Animal Husbandry and Veterinary Medicine, Beijing Vocational College Agriculture, Beijing 102442, P.R. China
Advance Journal of Food Science and Technology 2013 6:783-786
Received: February 25, 2013 | Accepted: March 27, 2013 | Published: June 05, 2013
Abstract
Ampicillin (AMP) was coupled with mcKLH by the method of EDC intermediate and injected into Balb/c mice as immunogen. The hybridomas were obtained by fusing mouse myeloma cells SP2/0 with splenocytes from the mice immunized with AMP-KLH. The chromosomal numbers were 97-104, the results indicated that the subclass of the McAb was IgG2a, the titer of ascitic fluid was 2×106, the affinity constant was 3×10-10 mol/L, the molecular weight was 154KD. The cross-reactivities of McAb to other antibiotics were below 0.01%. The linear detecting range of calibration curves to detect AMP was 0.5 ng/mL-100 ng/mL. The formular was Y = 0.0569X+0.1308, R2 = 0.9948. The limit of detection was 0.3 ng/mL.
Keywords:
Ampicillin, ELISA, monoclonal antibody,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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