Research Article | OPEN ACCESS
Liquid Culture of Adventitious Roots is a Potential Alternative to Field Cultivation for Psammosilene tunicoides, a Rare and Endangered Endemic Medicinal Plant
1, 2Zongshen Zhang, 2Zhenyan Yu, 1Zhaoxia Jin, 1Jun Liu and 1Yunfang Li
1Department of Biological Engineering, Dalian Polytechnic University, Qinggongyuan, Ganjingzi District, Dalian, 116034, P.R. China
2Henan Province Health Albert Med-Tech Co., Ltd, Zhengzhou, 450001, P.R. China
Advance Journal of Food Science and Technology 2013 2:127-131
Received: August 31, 2012 | Accepted: October 19, 2012 | Published: February 15, 2013
Abstract
The aim of this study was to establish an adventitious roots culture system for sterile plantlet segments of P. tunicoides and improved the accumulation of total saponins in cultured roots. Psammosilene tunicoides is a native Chinese plant with high commercial value as medicinal herb. Combination of NAA and IBA significantly affected the adventitious roots formation on agar-solided B5 media and a maximal induction rate of 83% was obtained at 24±2°C with a photoperiod of 12 h. With a shaking of 110 rpm in darkness, transferring the detached adventitious roots to the growth regulator free 1/2 B5 liquid media notably increased the biomass production compared to that on solid media over a 30-day-culture period. Further analyses showed that more saponins could be accumulated in the liquid culture than in the solid culture and the addition of exogenous oxalic acid to the liquid media could enhance the accumulation of total saponins in adventitious roots. These results suggested that adventitious roots culture will be an efficient alternative to the field cultivation of intact plants for the production of useful natural compounds from P. tunicoides.
Keywords:
Adventitious roots, liquid culture, Psammosilene tunicoides, saponin,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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