Research Article | OPEN ACCESS
Study on Seed Morphology and Genetic Diversity of Jatropha curcas L. from Different Provenances
1Junjiao Guan, 2Hong Yu, 1Jianhua Zhang, 3Roupeng Yang and 4Yuan-hong Fan
1Quanlity Standard and Testing Technology Research Institute, Yunnan Department of
Agriculture Sciences, Yunnan Kunming, 650223, China
2Yunnan Herbal Laboratory, Department of Herb Biotic Resources, Yunnan University,
Yunnan Kunming, 650091, China
3Department of Life Science and Technology, Honghe University, Yunnan Menzi, 661100, China
4Research Center of Biomass Energy, Yunnan Department of Agricultural Science,
Yunnan Kunming, 650223, China
Advance Journal of Food Science and Technology 2013 2:169-173
Received: September 14, 2012 | Accepted: November 08, 2012 | Published: February 15, 2013
Abstract
Jatropha curcas L., a multipurpose shrub has acquired significant economic importance for its seed oil which can be converted to biodiesel, is emerging as an alternative to petro-diesel. The present study aims at characterization of the seed morphology and genetic diversity of Jatropha curcas L. from eight different provenances for providing support for the breeding and allocation of seed. Five traits were investigated, including hundred seed weight, seed length, width, lateral diameter, seed length and width ratio. The genetic diversity of eight populations from different provenances was estimated using DALP method (5 primers). The results showed that seed morphology had significant variation among locations. Five DALP primers generated highly reproducible and stable DNA fragments. 219 of 244 loci were polymorphic, i.e., PPB was 89.75%. And POPGENE analysis indicated the total Nei’s gene diversity (H) was 0.2878, the total Shannon's Information index (I) was 0.4331, and the coefficient of Gene differentiation (Gst) was 0.8200 among populations, namely 82.00% genetic variation occurring among populations and 20.00% remaining within population. It was suggested that a high level of genetic variation should be occur among the different populations of J. curcas. The high genetic differentiation among the populations could be caused by not only the limited gene flow (Nm = 0.1097) but also the genetic drift. The result indicates that the seed morphology among populations showed some certain differentiation. The eight populations had high level of genetic diversity and show apparent genetic differentiation. So that provenance selection has great potentiality.
Keywords:
Different provenances, , genetic diversity, , Jatropha curcas L , seed morphology,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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