Research Article | OPEN ACCESS
Response Surface Methodology Used for Investigating Soluble Organic Selenium Accumulation in Yeasts
1, 3Yunfeng Ma, 2, 3Fu Xiangand and 3, 4Longjiang Yu
1Department of Life Science, Henan University, Kaifeng, 475004, China
2Department of Life Science and Engineering, Huanggang Normal
University, Huanggang, 438000, China
3Department of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology
4Key Laboratory of Molecular Biophysics, Ministry of Education, Wuhan, 430074, China
Advance Journal of Food Science and Technology 2013 2:174-179
Received: September 14, 2012 | Accepted: October 24, 2012 | Published: February 15, 2013
Abstract
Selenium-enriched yeasts were usually regarded as an ideal organic selenium source. The study investigated the Soluble Organic Selenium (SOS) accumulation in three species of yeasts: Saccharomyces cerevisiae, Candida rugosa and Candida utilis. The effects of main factors on organic selenium accumulation were evaluated. The Response Surface Methodology (RSM) and Central Composite Design (CCD) were applied to optimize the dosage of main factors in fermentation medium. SOS content in the dry cells of C. utilis (1.31±0.065 mg/g) was higher than that of S. cerevisiae (1.20±0.057 mg/g) and C. rugosa (0.68±0.033 mg/g). However, as far as the productivity of per unit volume of broth was concerned, Candida rugosa also accumulated the most SOS (4.81±0.18 mg/L) among the three species of yeasts. Higher content of SOS in yeasts inhibited the cell growth and led to decrease of SOS per unit volume during yeasts fermentation. Perhaps, yeasts with lower content of SOS have the advantage of SOS accumulation per unit volume broth accumulation.
Keywords:
Central Composite Design (CCD), optimal culture, organic selenium, Response Surface Methodology (RSM), selenium-enriched yeasts,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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