Research Article | OPEN ACCESS
Cloning of Formate dehydrogenase Gene and Effect on the Waterlogging Tolerance of Brassica napus L.
1, 2, 3Ben-bo Xu, 1Yong Cheng, 1Xiao Xi-ling Zou, 1Guang-yuan Lu, 1Pu-ying Zheng and 1Xue-kun Zhang
1Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, P.R. China
2Life Sciences College of Yangtze University
3Engineering Research Center of Wetland Agriculture in the Middle Reaches of the
Yangtze River, Ministry of Education, Jingzhou 434025, P.R. China
Advance Journal of Food Science and Technology 2013 4:440-444
Received: October 31, 2012 | Accepted: December 22, 2012 | Published: April 15, 2013
Abstract
A Formate dehydrogenase (FDH) gene, named BnFDH-1, was cloned from oilseeds (Brassica napus L.) by the Rapid Amplification of cDNA Ends (RACE) method. The Complementary DNA (cDNA) consisted of 1488 bp and had an open reading frame of 1140 bp encoding a polypeptide of 384 amino acids with a molecular weight of 42.3 kD and an isoelectric point of 8.17. BnFDH-1 showed high homology to known FDH genes, especially FDH from Arabidopsis thaliana. Conserved domain search and motif characterization identified BnFDH protein as Rossmann-fold NAD (P) (+)-binding proteins. NetPhos 2.0 search predicted 18 significant phosphorylation sites. To investigate the effect of BnFDH on waterlogging tolerance of B. napus, 12 B. napus cultivars with different waterlogging tolerance were used in the research and waterlogging Tolerance Index (WTI) was calculated by multiplying relative percentage germination and the relative seedling height. BnFDH-1 was cloned from the 12 materials also, but the results indicated all of them had same protein sequence. BnFDH expression level in 12 B. napus cultivates with different waterlogging tolerance after waterlogging treatment was measured. The results indicated expression level of BnFDH in 12 cultivates had significant difference. But correlation analysis proved that BnFDH expression level was uncorrelated with Waterlogging tolerance index (WTI). So it was not the BnFDH that was key gene for the waterlogging tolerance of B. napus.
Keywords:
Brassica napus L., cloning, formate dehydrogenase, waterlogging tolerance, waterlogging tolerance index,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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