Research Article | OPEN ACCESS
A Validated RP-LC Method for the Determination of Erythromycin an Oxime and Related Substances
1Zhiling Cao, 1Bing Liu, 1Weiwei Liu, 1Yinxing Su, 1Wenming Bo and 2Guowei Yao
1Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology,
Lianyungang, 222005, China
2Department of Life Science, Beijing Institute of Technology, Beijing 100081, China
Advance Journal of Food Science and Technology 2013 1:68-71
Received: October 09, 2012 | Accepted: November 23, 2012 | Published: January 15, 2013
Abstract
A liquid chromatographic method was developed and validated for the determination of erythromycin an oxime and related substances. The chromatographic analysis was performed on Inertsil C18 ODS column, using the phosphate buffer solution (0.02 M, pH 6.5)-acetonitrile (40:60, V/V) as the mobile phase. Wavelength of the UV detector was 215 nm. All four related substances, including Z isomer of erythromycin A oxime, erythromycin A, erythromycin A-6, 9-hemiketal and erythromycin A-6, 9-9, 12-spiroketal were separated and determined under isocratic elution. The effect of pH and the concentration of mobile phase on separation were studied. The developed method was successfully applied to the analysis of erythromycin an oxime in commercial formulation.
Keywords:
Column liquid chromatography, erythromycin an oxime, macrolide antibiotics, related substance,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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