Research Article | OPEN ACCESS
Application of Response Surface Methodology to Optimize Hydrolysis of Collagen from Croaker Scale
Y. Hong, W.W. Chen, Z.B. Jia and G.R. Huang
College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang, 310018, China
Advance Journal of Food Science and Technology 2013 12:1646-1651
Received: September 04, 2013 | Accepted: September 17, 2013 | Published: December 05, 2013
Abstract
Response Surface Methodology (RSM) based on a Central Composite Rotatable Design (CCRD) was applied to optimize the hydrolysis of collagen from croaker scale with the aim to maximize the Degree of Hydrolysis (DH). Effects of pH, temperature, the ratio of Enzyme to Substrate (E/S) and hydrolyzing time on DH were investigated. The results showed that the DH was predicted very well by a second-order polynomial model (R2 = 0.9153, p<0.0001). The highest DH was theoretically predicted to be 45.43%, under the optimal conditions (pH 8.0, 54.0 of temperature, 3.0% of E/S and 2.3 h of hydrolysis time). The recovery experimental DH under optimal conditions was calculated to be 46.63%, which confirmed the reliability of the method.
Keywords:
Central composite rotatable design, degree of hydrolysis, response surface methodology, scale collagen,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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