Abstract

Response Surface Methodology (RSM) based on a Central Composite Rotatable Design (CCRD) was applied to optimize the hydrolysis of collagen from croaker scale with the aim to maximize the Degree of Hydrolysis (DH). Effects of pH, temperature, the ratio of Enzyme to Substrate (E/S) and hydrolyzing time on DH were investigated. The results showed that the DH was predicted very well by a second-order polynomial model (R2 = 0.9153, p<0.0001). The highest DH was theoretically predicted to be 45.43%, under the optimal conditions (pH 8.0, 54.0 of temperature, 3.0% of E/S and 2.3 h of hydrolysis time). The recovery experimental DH under optimal conditions was calculated to be 46.63%, which confirmed the reliability of the method.

Keywords:

Central composite rotatable design, degree of hydrolysis, response surface methodology, scale collagen,

References

Competing interests

The authors have no competing interests.

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The authors have no competing interests.