Abstract

Black garlic was created by keeping whole ordinary garlic in a humidity controlled room at 70-80°C for 10-15 days without any artificial treatments and additives. The black garlic was extracted with 80% ethanol and concentrated to obtain black garlic ethanol extracts. The saccharides of the extracts were analyzed according to the method of Phenol Sulphate colorimetry, DNS and High Performance Liquid Chromatography (HPLC). The antioxidant capacity in vitro of the extracts was assessed by measuring the scavenging activities on 1, 1-Diphenyl-2- Picrylhydrazyl (DPPH). Drosophila melanogaster were employed to explore the effect of black garlic extract on lifespan and antioxidant index in vivo. The results showed that the total sugar content in the black garlic extracts was 55.5% including monosaccharide, disaccharide and polysaccharide, the content of reducing sugar was 25.22%. The results from this study demonstrated black garlic extracts possessed strong antioxidant capacity in vitro in a dose- dependent manner, the longevity of Drosophila melanogaster treated with black garlic extract was prolonged evidently and the content of MDA was decreased by improving SOD (including CuZn-SOD and Mn-SOD) and CAT activities.

Keywords:

Antioxidant, black garlic, Drosophila, saccharides,

References

1. Boyd, O., P. Weng, X. Sun, T. Alberico, M. Laslo and et al., 2011. Nectarine promotes longevity in Drosophila melanogaster. Free Radical Bio Med., 50(11): 669-1678.
   CrossRef    PMid:21406223 PMCid:PMC3090488    
2. Chao, L.F., H. Guo and Z. Li, 2012. Research progress in the nutritional value and health effects of black garlic. Sci. Technol. Food Ind., 33(13): 429-432.
   
3. Kim, J.H., S.H. Nam, C.W. Rico and M.Y. Kang, 2012. A comparative study on the antioxidative and anti-allergic activities of fresh and aged black garlic extracts. Int. J. Food Sci. Tech., 47: 1176-1182.
   CrossRef    
4. Kim, I., J.Y. Kim, Y.J. Hwang, K.A. Hwang, A.S. Om et al., 2011a. The beneficial effects of aged black garlic extract on obesity and hyperlipidemia in rats fed a high-fat diet. J. Med. Plants Res., 5: 3159-3168.
   
5. Kim, M.H., M.J. Kim, J.H. Lee, J.I. Han, J.H. Kim et al., 2011b. Hepatoprotective effect of aged black garlic on chronic alcohol-induced liver injury in rats. J. Med. Food, 14(7-8): 732-738.
   CrossRef    PMid:21663494    
6. Lee, E.N., Y.W. Choi, H.K. Kim, J.K. Park, H.J. Kim, et al., 2011. Chloroform extract of aged black garlic attenuates TNF-a-induced ROS generation, VCAM-1 expression, NF-kB activation and adhesiveness for monocytes in human umbilical vein endothelial cells. Phytother. Res., 25: 92-100.
   CrossRef    PMid:20623600    
7. MacDonald-Wicks, L.K., L.G. Wood and M.L. Garg, 2006. Methodology for the determination of biological antioxidant capacity in vitro: A review. J. Sci. Food Agr., 86: 2046-2056.
   CrossRef    
8. Masuko, T., A. Minami, N. Iwasaki, T. Majima, S.I. Nishimura and Y.C. Lee, 2005. Carbohydrate analysis by a phenol-sulfuric acid method in microplate format. Anal. Biochem., 339: 69-72.
   CrossRef    PMid:15766712    
9. Noctor, G., S.V. Jovanovic and C.H. Foyer, 2000. Peroxide processing in photosynthesis: Antioxidant coupling and redox signalling. Philos. T. Roy. Soc. B., 355: 1465-1475.
   CrossRef    PMid:11128000 PMCid:PMC1692876    
10. Purev, U., M.J. Chung and D.H. Oh, 2012. Individual differences on immunostimulatory activity of raw and black garlic extract in human primary immune cells. Immunopharm. Immunot., 34(4): 651-660.
    
11. Sasaki, J., C. Lu, E. Machiya, M. Tanahashi and K. Hamada, 2007. Processed black garlic (Allium sativum) extracts enhance anti-tumor potency against mouse tumors. Med. Aromatic Plant Sci. Biotech., 1(2): 278-281.
    Direct Link
12. Sato, E., M. Kohno, H. Hamano and Y. Niwano, 2006. Increased anti-oxidative potency of garlic by spontaneous short-term fermentation. Plant Food Hum. Nutr., 61: 157-160.
    CrossRef    PMid:17075725    
13. Seo, Y.J., O.C. Gweon, J.E. Im, Y.M. Lee, M.J. Kang and et al., 2009. Effect of garlic and aged black garlic on hyperglycemia and dyslipidemia in animal model of type 2 diabetes mellitus. J. Food Sci. Nutr., 14(1): 1-7.
    CrossRef    
14. Sun, V.I., W.O. Larry and U. Ving, 1988. A simple method for clinical assay of superoxide dismutase. Clin. Chem., 34(3): 497-500.
    PMid:3349599    
15. Wang, D.N., Y.H. Feng, J. Liu, J.Z. Yan, M.R. Wang, J.I. Sasaki and et al., 2010. Black garlic (Allium sativum) extracts enhance the immune system. Med. Aromatic Plant Sci. Biotech., 4: 37-40.
    
16. Wang, X., F. Jiao, Q.W. Wang, J. Wang, K. Yang and et al., 2012. Aged black garlic extract induces inhibition of gastric cancer cell growth in vitro and in vivo. Mol. Med. Rep., 5: 66-72.
    PMid:21922142    
17. Yen, G.C. and P.P. Hsieh, 1995. Antioxidative activity and scavenging effects on active oxygen of xylose-lysine Maillard reaction products. J. Sci. Food Agr., 67: 415-420.
    CrossRef    
18. Zhao, K., P. Xue and G. Gu, 2008. Study on determination of reducing sugar content using 3, 5-dinitrosalicylic acid method. Food Sci., 29(08): 534-536.
    
19. Zhu, B., H. Wu, Y. Liu and J. Tun and Z. Yao, 2008. The antioxidation of black garlic (In Chinese). Food Res. Dev., 29(10): 58-60.
    

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