Research Article | OPEN ACCESS
Optimized Preparation of Catechin Nanoliposomes by Orthogonal Design and Stability Study
1Xiaobo Luo, 1Rongfa Guan, 2Xiaoqiang Chen, 1Mingqi Liu, 3Yunbin Hao and 1Han Jiang
1Zhejiang Provincial Engineering Laboratory of Quality Controlling Technology and Instrumentation for Marine Food, China Jiliang University, Hang-Zhou, 310018, China
2School of Food and Pharmaceutical Engineering, Hubei University of Technology,
Wuhan, 430068, China
3Zhoushan Fishery Testing Center, Zhoushan 316111, China
Advance Journal of Food Science and Technology 2014 7:921-925
Received: April ‎26, ‎2014 | Accepted: May ‎25, ‎2014 | Published: July 10, 2014
Abstract
Catechin nanoliposomes are prepared by reverse-phase evaporation method. Taking the entrapment efficiency of catechin nanoliposomes as the main evaluation index, the prescription of the catechin nanoliposomes was optimized by orthogonal design. Found by experiment, the orthogonal design test showed that phosphatidylcholine to cholesterol ratio had the highest influence in preparation and rotary evaporation temperature the lowest, with catechin concentration and ion strength in between. The best conditions of preparation of catechin nanoliposomes are that phosphatidylcholine to cholesterol ratio of 4, catechin concentration of 4.00 mg/mL, ion strength of 0.02 mol/Landrotary evaporationtemperature of 40°C, encapsulation efficiency can reach about 53.69±1.65%, the particle size is 235±4 nm. Finally, using pH, leakage ratio and size to explore the stability, catechin nanoliposomes showed an acceptable stability
Keywords:
Catechin, entrapment efficiency, nanoliposomes, orthogonal design, particle size, stability,
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Competing interests
The authors have no competing interests.
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