Research Article | OPEN ACCESS
Mutation Breeding of Salt-tolerant and Ethanol-producing Strain S. cerevisiae H058 by Low-energy Ion Implantation
1, 2Shoubao Yan, 1Shunchang Wang, 2Zhijun Zhai, 2Xiangsong Chen and 2Jinyong Wu
1School of Life Science, Huainan Normal University, Huainan, Anhui 232001
2Key Laboratory of Ion Beam Bio-Engineering of Institute of Plasma Physics, Chinese Academy of Sciences, Hefei Anhui 230031, People's Republic of China, China
Advance Journal of Food Science and Technology 2014 7:941-946
Received: May ‎19, ‎2014 | Accepted: June ‎18, ‎2014 | Published: July 10, 2014
Abstract
To obtain an industrial strain with high ethanol fermentation efficiency under salted conditions, the wild strain H058 of Saccharomyces cerevisiae was mutated by means of nitrogen ions implantation. Mutagenic effects of strain H058 by low energy N+ ion implantation were studied.A similar "saddle shape" survival curve due to ion beam irradiation appeared again in this study. By repeated screening, a high salt-tolerant and ethanol-producing strain M158 was obtained. Results showed that in medium contained 0, 1.5, 3.0, 4.5, 6.0% NaCl, M158 produced maximal ethanol of 98.3, 97.2, 96.4, 95.6 and 78.3 g/L at 54, 54, 54 and 72 h, respectively. However, the original strain H058 maximal ethanol of 95.2, 90.9, 84.8, 79.4 and 67.5 g/L at 60, 60, 66 and 72 h, respectively. In addition, the ethanol yield (g/g) in all of the NaCl concentrations for M158 is 0.492, 0.486, 0.482, 0.48 and 0.392 g/g, respectively, which were higher than those (0.476, 0.455, 0.424, 0.397 and 0.338 g/g, respectively) of the original strain H058. The higher production and shorter fermentation period suggest that strain M158 is a good salt-tolerant and ethanol-producing strain.
Keywords:
Breeding, ethanol fermentation, ion implantation, salt-tolerant,
References
- Limtong, S., T. Seki and H. Taguchi, 1986. Flocculation of Saccharomyces cerevisiae AM12. Ann. Rep. IC. Biotech., 9: 335-340.
- Ma, M. and Z.L. Liu, 2010. Mechanisms of ethanol tolerance in Saccharomyces cerevisiae. Appl. Microbiol. Biot., 87: 829-845.
CrossRef PMid:20464391
- Miller, G.L., 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem., 31(3): 426-428.
CrossRef
- Pang, M., J. Yao and D. Wang, 2009. A study of mutation breeding of high-yielding tryptophanase Escherichia coli by low-energy N+ ion beam implantation. Plasma Sci. Technol., 11(6): 744-749.
CrossRef
-
Savitree, L., D. Somkid, Y. Wichien and S. Wilia, 1998. Construction of high ethanol fermenting halotolerant hybrid by intergeneric protoplast fusion of Saccharomyces cerevisiae and Zygosaccharomyces rouxii. Kasetsart J. Nat. Sci., 32(2): 213-223.
- Shao, C.L., A. Xu and Z.L. Yu, 1997. Charge exchange effect of ion implantation to biomolecules. Nucl. Technol., 20: 70-73, (In Chinese).
- Shao, C.L. and Z.L. Yu, 1997. Mass deposition in tyrosine irradiated by a N+ ion. Radiat. Phys. Chem., 50(6): 595-599.
CrossRef
- Tajima, K. and H. Yashizumi, 1975. Mechanisms of abnormal fermentation of distiller's yeast in salted media (such as molasses media) from the point of NAD (P) redox balances. J. Ferment. Technol., 53: 841-853.
- Tanaka, A., A. Sakamoto, Y. Ishigaki, O. Nikaido, G. Sun, Y. Hase, N. Shikazono, S. Tano and H. Watanabe, 2002. An ultraviolet-B-resistant mutant with enhanced DNA repair in Arabidopsis. Plant Physiol., 129(1): 64-71.
CrossRef PMid:12011338 PMCid:PMC155871
- Wu, L.F. and Z.L. Yu, 2001. Radiobiological effects of a low-energy ion beam on wheat. Radiat. Environ. Bioph., 40: 53-57.
CrossRef PMid:11357711
- Wu, L.J., R.P. Gerhard, A. Xu, C.A. Waldren, C.R. Geard, Z.L. Yu and T.K. Hei, 1999. Targeted cytoplasmic irradiation with alpha particles induces mutations in mammalian cells. P. Natl. Acad. Sci. USA, 96: 4959-4964.
CrossRef PMid:10220401
- Xu, A., J. Yao, L. Yu, S. Lv, J. Wang, B. Yan and Z. Yu, 2004. Mutation of Luconobacter oxydans and Bacillus megaterium in a two-step process of L-ascorbic acid manufacture by ion beam. J. Appl. Microbiol., 96: 1317-1323.
CrossRef PMid:15139924
-
Yang, Y., Y. Fan, W. Li, D. Wang, Y. Wu, Z. Zheng and Z. Yu, 2007. Optimization of L (+)-lactic acid production from xylose with Rhizopus oryzae mutant RLC41-6 breeding by low-energy ion implantation. Plasma Sci. Technol., 9(5): 638-642.
CrossRef
- Yu, J.L., X. Zhang and T.W. Tan, 2009a. Optimization of media conditions for the production of ethanol from sweet sorghum juice by immobilized Saccharomyces cerevisiae. Biomass. Bioenerg., 33: 521-526.
CrossRef
-
Yu, L.D., T. Kamwanna and I.G. Brown, 2009b. The low-energy ion range in DNA. Phys. Med. Biol., 54(16): 5009-5022.
CrossRef PMid:19652287
- Yu, Z.L., 2000. Ion beam application in genetic modification. IEEE T. Plasma. Sci., 28(1): 128-132.
CrossRef
- Yu, Z.L., J.J. He, J.J. He and J.G. Deng, 1989. Preliminary studies on the mutagenic mechanism of the ion implantation on rice. AnHui Agric. Sci., 28(1): 12-16, (In Chinese).
-
Yu, Z.L., J.J. He, J.B. Yang, Y.J. Wu, B.J. Chen, J. Zhou, Y.Q. Chen, H.K. Sun and Z.Q. Yin, 2000. Patent No. ZL93103361.6, People's Republic of China.
- Zhang, X., B. Lin, X. Gao and H. Shen, 2010. Enhancement of wanlongmycin production by nitrogen ion beam implantation. Electron. J. Biotechn., 13(1): 1-6.
- Zhu, G. and Z. Wang, 1992. Laboratory Technical Handbook of Industry Microorganisms. 1st Edn., China Light Industry Press, Jiangsu, China, (In Chinese).
Competing interests
The authors have no competing interests.
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