Abstract

This present study is based on the single factor test and the significant factors of emulsification conditions, which influence the process of microencapsulation of B. bifidum BB28 and BB01, will be screen out by Plackett-Burman experimental. Bifidobacterium BB0I and BB28 were encapsulated by using emulsion method respectively. Several factors, such as alginate concentration, cell suspension-alginate ratios, CaCl ₂ concentration, Tween 80 concentration, emulsification time, encapsulating time and oil-water ratios have been investigated, the results showed that sodium alginate concentration, cell suspension-alginate ratios, encapsulating time and emulsification time have a significant effect for Bifidobacterium BB01 microcapsules; CaCl₂ concentration, cell suspension-alginate ratios, sodium alginate concentration and emulsification time have a significant effect for Bifidobacterium BB28 microcapsules.

Keywords:

Bifidobacterium bifidum, emulsion, microencapsulation, plackett-burman design,

References

1. Brinques, G.B. and M.A.Z. Ayub, 2011. Effect of microencapsulation on survival of Lactobacillus plantarum in simulated gastrointestinal conditions, refrigeration and yogurt. J. Food Eng., 103: 123-128.
   CrossRef    
2. Chakravarti, R. and V. Sahai, 2002. Optimization of compactin production in chemically defined production medium by Penicillium citrinum using statistical methods. Process Biochem., 38: 481-486.
   CrossRef    
3. Charteris, W.P., P.M. Kelly, L. Morelli and J.K. Collins, 1998. Development and application of an in vitro methodology to determine the transit tolerance of potentially probiotic Lactobacillus and Bifidobacterium species in the upper human gastrointestinal tract. J. Appl. Microbiol., 84: 759-768.
   CrossRef    PMid:9674129    
4. Collado, C.M. and Y. Sanz, 2006. Method for direct selection of potentially probiotic Bifidobacterium strains from human feces based on their acid-adaptation ability. J. Microbiol. Meth., 66: 560-563.
   CrossRef    PMid:16522337    
5. Doleyres, Y., I. Fliss and C. Lacroix, 2004. Increased stress tolerance of Bifidobacterium longum and Lactococcus lactis produced during continuous mixed-strain immobilized-cell fermentation. J. Appl. Microbiol, 97: 527-539.
   CrossRef    PMid:15281933    
6. Filomena, N., O. Pierangeo, F. Florinda and C. Raffaele, 2012. Microencapsulation in food science and biotechnology. Curr. Opin. Biotech., 23: 182-186.
   CrossRef    PMid:22024623    
7. Fritzen-Freire, C.B., C.M.O. Müller, J.B. Laurindo and E.S. Prudêncio, 2010. The influence of Bifidobacterium Bb-12 and lactic acid incorporation on the properties of Minas Frescal cheese. J. Food Eng., 96: 621-627.
   CrossRef    
8. Gardiner, G., E. O'Sullivan, J. Kelly, M.A.E. Auty, G.F. Fitzgerald, J.K. Collins, R.P. Ross and C. Stanton, 2000. Comparative survival rates of human-derived probiotic Lactobacillus paracasei and L. salivarius strains during heat treatment and spray drying. Appl. Environ. Microb., 66: 2605-2612.
   CrossRef    PMid:10831444 PMCid:PMC110587    
9. Gouin, S., 2004. Microencapsulation: Industrial appraisal of existing technologies and trends. Trends Food Sci. Tech., 15: 330-347.
   CrossRef    
10. He, C., W. Ye, S. Guowei and J. Yali, 2012. Effect of alginate and cell suspension on viable counts and efficacy of entrapment of encapsulated B. bifidum BB28. Adv. Mater. Res., 531: 499-502.
    
11. Kailasapathy, K., 2006. Survival of free and encapsulated probiotic bacteria and their effect on the sensory properties of yoghurt. Food Sci. Technol., 39: 1221-1227.
    
12. Matsumoto, M., H. Ohishi and Y. Benno, 2004. H+-ATPase activity in Bifidobacterium with special reference to acid tolerance. Int. J. Food Microbiol., 93: 109-113.
    CrossRef    PMid:15135587    
13. Mohammadi, R., A.M. Mortazavian, R. Khosrokhava and A.G. Cruz, 2011. Probiotic ice cream: Viability of probiotic bacteria and sensory properties. Ann. Microbiol., 61: 411-424.
    CrossRef    
14. Patel, R.R. and J.K. Patel, 2010. Novel technologies of oral controlled release drug delivery system. Syst. Rev. Pharm., 1: 128-132.
    
15. Ribeiro, A.J., R.J. Neufeld, P. Arnaud and J.C. Chaumeil, 1999. Microencapsulation of lipophilic drugs in chitosan-coated alginate microspheres. Int. J. Pharm., 187: 115-123.
    CrossRef    
16. Sanders, M.E., 2003. Probiotics: Considerations for human health. Nutr. Rev., 61: 91-99.
    CrossRef    PMid:12723641    
17. Pinto, S.S., C.B. Fritzen-Freire, I.B. Mu?oz, P.L.M. Barreto, E.S. Prud?ncio and R.D.M.C. Amboni, 2012. Effects of the addition of microencapsulated Bifidobacterium BB-12 on the properties of frozen yogurt. J. Food Eng., 111: 563-569.
    CrossRef    
18. Takahashi, N., J.Z. Xiao, K. Miyaji, T. Yaeshiima, A. Hiramatsu and K. Iwatsuki, 2004. Selection of acid tolerant bifidobacteria and evidence for a low-pH-inducible acid tolerance response in Bifidobacterium longum. J. Dairy Res., 71: 340-345.
    CrossRef    PMid:15354581    
19. Thomas, H., F. Petra and K. Ulrich, 2009. Microencapsulation of probiotic cells by means of rennet-gelation of milk proteins. Food Hydrocolloid., 23: 160-167.
    CrossRef    
20. Xiao, Y.L., G.C. Xi, W.S. Zhong, J.P. Hyun and S.C. Dong, 2011. Preparation of alginate/ chitosan/carboxymethyl chitosan complex microcapsules and application in Lactobacillus casei ATCC 393. Carbohyd. Polym., 83: 1479-1485.
    CrossRef    

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