Research Article | OPEN ACCESS
Optimized Ultrasonic-assisted Extraction of Flavonoids from Pyracantha fortuneana Fruit and Evaluation of Antibacterial Activities
1Yun Zhang, 1, 2Wei Wu, 1Xin Yu, 1Anju Xiao and 1Jing Lin
1School of Medicine, Hubei Polytechnic University
2Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention, Huangshi CN-435003, P.R. China
Advance Journal of Food Science and Technology 2014 12:1301-1306
Received: June ‎11, ‎2014 | Accepted: July ‎19, ‎2014 | Published: December 10, 2014
Abstract
Ultrasonic-Assisted Extraction (UAE) method was developed for extraction of total flavonoids from Pyracantha fortuneana fruit. The UAE parameters and the antioxidant activities of flavonoids extract were investigated. Important variables and their levels were obtained using single factor analysis method and central composite design. Through Response Surface Methodology (RSM) design experiments, the processing conditions were optimized as follows: ethanol concentration, 81.15%; liquid-solid ratio, 30.00 mL/g; extraction time, 3.14 h; and temperature, 69.55°C. Under the optimum condition, the extraction yield was 1.261%, which was well matched with the predicted values of 1.263%. The antibacterial activities of extract against four bacterial strains were determined by agar well diffusion method and the results indicated that the extract showed potent antibacterial activities, which concludes its application as natural antibacterial agent.
Keywords:
Antibacterial activities, central composite design, flavonoids, Pyracantha fortuneana, response surface methodology,
References
- Aladesanmi, A.J., A. Sofowora and J.D. Leary, 1986. Preliminary biological and phytochemical investigation of two Nigerian medicinal plants. Pharm. Biol., 24: 147-153.
CrossRef - Bimakr, M., R.A. Rahman, F.S. Taip, A. Ganjloo, L.M. Salleh, J. Selamat, A. Hamid and I.S.M. Zaidul, 2011. Comparison of different extraction methods for the extraction of major bioactive flavonoid compounds from spearmint (Mentha spicata L.) leaves. Food Bioprod. Process., 89: 67-72.
CrossRef
- Box, G.E. and K.G. Wilson, 1951. On the experimental attainment of optimum conditions. J. Roy. Stat. Soc. B Mat., 13: 1-45.
CrossRef
-
Dall'Agnol, R., A. Ferraz, A.P. Bernardi, D. Albring, C. Nör, L. Sarmento, L. Lamb, M. Hass, G. von Poser and E.E.S. Schapoval, 2003. Antimicrobial activity of some Hypericum species. Phytomedicine, 10: 511-516.
CrossRef PMid:13678236
-
Deans, S.G. and G. Ritchie, 1987. Antibacterial properties of plant essential oils. Int. J. Food Microbiol., 5: 165-180.
CrossRef
- Deng, R.F., S.E. Wang and G.R. Li, 1990. Studies on the nutritional components of the fruit of the wild plant-firethorn. Acta Nutrimenta Sinica, 12: 79-84.
-
El-Abyad, M.S., N.M. Morsi, D.A. Zaki and M.T. Shaaban, 1990. Preliminary screening of some Egyptian weeds for antimicrobial activity. Microbios, 62(250): 47-57.
PMid:2336037
-
Grigonis, D., P.R. Venskutonis, B. Sivik, M. Sandahl and C.S. Eskilsson, 2005. Comparison of different extraction techniques for isolation of antioxidants from sweet grass (Hierochloë odorata). J. Supercrit. Fluid., 33: 223-233.
CrossRef
-
Hou, J.J., H. Xue, Y.S. Li, X.L. Liu and W.K. Wei, 2002. Effects of Pyracantha on plasma lipid and blood rheology of rats fed a high fat diet. China J. Public Health, 18: 1059-1060.
- Hou, J.J., W.K. Wei, H. Huang and M.G. Wu, 2003. Antioxidation effects of Pyracantha on aging mice model induced by overdose of d-galactuse. Chinese J. Public Health, 19: 944-945.
- Li, C., Y. Ge, D. Wan, J. Hu, C. Ying and L. Wang, 2011. Optimization of extraction condition and quantification of total flavonoids in Elaeagni Folium. Pharmacogn. J., 3: 8-12.
CrossRef
- Liu, W., Y. Yu, R. Yang, C. Wan, B. Xu and S. Cao, 2010. Optimization of total flavonoid compound extraction from Gynura medica leaf using response surface methodology and chemical composition analysis. Int. J. Mol. Sci., 11: 4750-4763.
CrossRef PMid:21151469 PMCid:PMC3000113
-
Ma, C.H., S.Y. Wang, L. Yang, Y.G. Zu, F.J. Yang, C.J. Zhao, L. Zhang and Z.H. Zhang, 2012. Ionic liquid-aqueous solution ultrasonic-assisted extraction of camptothecin and 10-hydroxycamptothecin from Camptotheca acuminata samara. Chem. Eng. Process., 57: 59-64.
CrossRef
- Myers, R.H. and D.C. Montgomery, 2002. Response Surface Methodology: Process and Product Optimization using Designed Experiments. 2nd Edn., John and Wiley, New York.
- Turker, A.U., A.B. Yildirim and F.P. Karakas, 2012. Antibacterial and antitumor activities of some wild fruits grown in Turkey. Biotechnol. Biotec. Eq., 26: 2765-2772.
CrossRef
- Van Gelder, C.W., W.H. Flurkey and H.J. Wichers, 1997. Sequence and structural features of plant and fungal tyrosinases. Phytochemistry, 45: 1309-1323.
CrossRef
-
Zhang, G., L. He and M. Hu, 2011. Optimized ultrasonic-assisted extraction of flavonoids from Prunella vulgaris L. and evaluation of antioxidant activities in vitro. Innov. Food Sci. Emerg., 12: 18-25.
CrossRef
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
|
|
![](http://www.maxwellsci.com/images/AJFST-maxw.jpg) |
ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
![Submit Manuscript](../images/sub.jpg) |
Information |
|
|
|
Sales & Services |
|
|
|