Research Article | OPEN ACCESS
Effective and Low-cost Purification of Lysozyme by Combination of Conventional Processes
1Yunfeng Ma, 2Kuiling Xue, 1Li Li, 1Shibin He and 1Fude Shang
1College of Life Science, Henan University
2Hospital of Henan University, Kaifeng, 475004, P.R. China
Advance Journal of Food Science and Technology 2014 4:517-519
Received: January 07, 2014 | Accepted: January 24, 2014 | Published: April 10, 2014
Abstract
In this study, a simple method to purify lysozyme was introduced, based on combination of conventional processes, including thermal treatment is electric point precipitation and gel chromatography. The sample was purified well by compared with lysozyme standard and no other proteins appeared in Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE).
Keywords:
Gel chromatography, isoelectric point precipitation, lysozyme, purification, thermal treatment,
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Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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