Abstract

With the development of molecular biotechnology, methods for identification of marine food are developed from protein to DNA. The experimental materials are striped bass, Sturgeon and mandarin fish. They were processed with boiling, salting and microwave-heating methods. DNA-based research has been conducted in almost every corner in food material identification. This study is mainly about the identification methods of distinguishing processed aquatic products and the influence of processing on the DNA integrity in the raw materials of marine foods.

Keywords:

DNA integrity, marine foods, processing,

References

1. Carrera, E., T. Garcia, A. Cespedes, I. Gonzalez, A. Fernandez, L.M. Asensio, P.E. Hernandez and R. Martin, 2000. Identification of smoked Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) using PCR-restriction fragment length polymorphism of the p53 gene. J. AOAC Int., 83(2): 341-346.
   PMid:10772171    
2. Chapela, M.J., C.G. Sotelo, P. Calo-Mata, R.I. Pérez-Martín, H. Rehbein et al., 2002. Identification of cephalopod species (Ommastrephidae and Loliginidae) in seafood products by forensically informative nucleotide sequencing (FINS). J. Food Sci., 67(5): 1672-1676.
   CrossRef    
3. Chapela, M.J., C.G. Sotelo, R.I. Perez-Martin, M.A. Pardo, B. Perez-Villareal, P. Gilardi and J. Riese, 2007. Comparison of DNA extraction methods from muscle of canned tuna for species identification. Food Control, 18(10): 1211-1215.
   CrossRef    
4. Clarke, S.C., J.E. Magnussen, D.L. Abercrombie, M.K. McAllister and M.S. Shivji, 2006. Identification of shark species composition and proportion in the Hong Kong shark fin market based on molecular genetics and trade records. Conserv. Biol., 20(1): 201-211.
   CrossRef    PMid:16909673    
5. Cozzohno, A.D. and I. Murray, 2004. Identification of animal meat muscles by visible and near infrared reflectance spectroscopy. LWT-Food Sci. Technol., 37: 447-452.
   
6. Downey, G., J. Mcelhinney and T. Feam, 2000. Species identification in selected raw homogenized meats by reflectance spectroscopy in the mid-infrared, near-infrared and visible ranges [J]. Appl. Spectrosc., 54(6): 894-899.
   CrossRef    
7. Hird, H.J., G.L. Hold, J. Chisholm, P. Reece, V.J. Russell, J. Brown, R. Goodier and R. MacArthur 2005. Development of a method for the quantification of haddock (Melanogrammus aeglefinus) in commercial products using real-time. PCR. Eur. Food Res. Technol., 220(5-6): 633-637.
   CrossRef    
8. Kochzius, M., M. Nölte, H. Weber, N. Silkenbeumer, S. Hjörleifsdottir, G.O. Hreggvidsson et al., 2008. DNA microarrays for identifying fishes. Mar. Biotechnol., 10(2): 207-217.
   CrossRef    PMid:18270778 PMCid:PMC2263118    
9. Lowenstein, J.H., G. Amato and S.O. Kolokotronis, 2009. The real maccoyii: Identifying tuna sushi with DNA barcodes-contrasting characteristic attributes and genetic distances [J]. PLoS One, 4(11): 7866.
   CrossRef    PMid:19924239 PMCid:PMC2773415    
10. Moran, P. and E. Garcia-Vazquez, 2006. Identification of highly prized commercial fish using a PCR-based methodology. Biochem. Mol. Biol. Edu., 34(2): 121-124.
    CrossRef    PMid:21638653    
11. Moretti, V.M., G.M. Turchini, F. Bellagamba and F. Caprino, 2003. Trace ability issues in fishery and aquaculture products. Vet. Res. Commun., 27: 497-505.
    CrossRef    PMid:14535457    
12. Rehbein, H., C.G. Sotelo, R.I. Perez-Martin, M.J. Chapela-Garrido, G.L. Hold, V.J. Russell, S. Pryde, A.T. Santos, C. Rosa, J. Quinteiro and M. Rey-Mendez, 2002. Differentiation of raw or processed eel by PCR-based techniques: Restriction fragment length polymorphism analysis (RFLP) and single strand conformation polymorphism analysis (SSCP). Eur. Food Res. Technol., 214(2): 171-177.
    CrossRef    
13. Santaclara, F.J., A.G. Cabado and J.M. Vieites, 2006. Development of a method for genetic identification of four species of anchovies: E. encrasicolus, E. anchoita, E. ringens and E. japonicus. Eur. Food Res. Technol., 223(5): 609-614.
    CrossRef    
14. Yancy, H.F., T.S. Zemlak, J.A. Mason, J.D. Washington, B.J. Tenge, N.T. Nguyen, J.D. Barnett, W.E. Savary, W.E. Hill, M.M. Moore, F.S. Fry, S.C. Randolph, P.L. Rogers and P.D.N. Hebert, 2008. Potential use of DNA barcodes in regulatory science: Applications of the regulatory fish encyclopedia. J. Food Prot., 71(1): 210-217.
    CrossRef    PMid:18236687    

Competing interests

The authors have no competing interests.

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