Research Article | OPEN ACCESS
Anti-hepatocarcinoma Effects of a Food Additive Chrysin Nanosuspension against Human HepG2 Cells
1Zhi-ping Wang, 2Qun Zhou, 3Hui-zhe Shi, 3Sheng Xiong and 3Yi-fei Wang
1School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China
2Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, China
3Institute of Biological Medicine, Jinan University, Guangzhou 510632, China
Advance Journal of Food Science and Technology 2015 8:627-630
Received: October 10, ‎2014 | Accepted: November ‎3, ‎2014 | Published: March 15, 2015
Abstract
Hepatocarcinoma, a malignant cancer, threaten human life badly. It is a current issue to seek the effective natural remedy from plant to treat cancer due to the resistance of the advanced hepatocarcinoma to chemotherapy. Chrysin (Chr), a major symbol ingredient in Chinese Propolis, has a wide range of pharmacological properties and is considered to have anti-hepatocarcinoma effects. However its low oral bioavailability restricts its wide application. In this report, Chr-Nanosuspension (Chr-NS) composed of Chr and poloxamer 188 was prepared by high pressure homogenization technique. The in vitro anti-hepatocarcinoma effects of Chr-NS relative to efficacy of bulk Chr were evaluated. The particle size and zeta potential of Chr-NS were 291.1 nm and -28.7 mV, respectively. MTT assay showed that Chr-NS effectively inhibited the proliferation of HepG2 cells and the corresponding IC50 values of Chr-NS and bulk Chr were 1.55 and 3.76 μg/mL. These results suggest that the delivery of Chr-NS is a promising approach for treating tumors.
Keywords:
Antitumor activity, chrysin, cytotoxicity, food additive, HepG2 cells, nanosuspension,
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Competing interests
The authors have no competing interests.
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