Abstract

Exopolysaccharide (EPS) produced from lactic acid bacteria has many bioactivities and the role of prebiotic as reported. However, being a well-knowen probiotic, there is little report about EPS-producing bifidobacteria. In this study, several bifidobacteria strains were isolated from baby feces with mupirocin lithium salt improved medium and evaluated for the production of EPS. Culturing strains with MRS medium containing 2% sucrose, one EPS-producing bifidobacteria strain A19 was screened by ethanol precipitation, dialysis, phenol-sulfuric acid method and other methods. By the physiological and biochemical characteristics, 16S rDNA and tuf gene sequence analysis, strain A19 was identified as Bifidobacterium pseudocatenulatum. It was a novel EPS-producing bifidobacteria and the EPS yield was 55.546 mg/L in MRS broth with 2% sucrose. It could be a potential available strain for microecologic products and food starters.

Keywords:

Bifidobacteria, exopolysacharide, identification, screening,

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The authors have no competing interests.

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