Research Article | OPEN ACCESS
Application of Bio-speckle Activity to Assess Seed Viability
Sen Men, Lei Yan and Hua Qian
School of Technology, Beijing Forestry University, Beijing, China
Advance Journal of Food Science and Technology 2015 3:214-218
Received: December 10, 2014 | Accepted: January 19, 2015 | Published: May 15, 2015
Abstract
This study presents an assessment method for seed viability, using bio-speckle technique. Bio-speckle is caused by moving of the biological material under highly coherent light. If this phenomenon can be measured by successive speckle patterns during the period of germination, it is possible to identify different activities of the seeds. Viable and non-viable pisumsativum seeds were illuminated by a helium-neon laser source of 7mW with wavelength of 632.8 nm. The speckle patterns were recorded by a digital colour charge-couple device camera and stored in the host computer for further analysis using Matlab. Two methods were used to obtain information of biological activities from these speckle patterns. It was observed that the seeds activities can be distinguished as viable seeds and non-viable seeds. The results indicate that bio-speckle can be used to assess seed viability.
Keywords:
Bio-speckle, dynamic speckle, laser speckle, seed viability, viability assessment,
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Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
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The authors have no competing interests.
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