Home            Contact us            FAQs
    
      Journal Home      |      Aim & Scope     |     Author(s) Information      |      Editorial Board      |      MSP Download Statistics

     Advance Journal of Food Science and Technology

Research Article   |   OPEN ACCESS

Determination of Sucrose and Glucosylglycerol in Intracellular Extracts of Cyanobacteria by Anion Exchange Chromatography

1, 2Xin Wang, 2Yun Fa, 3Shengfeng Le, 4, 5Yangkai Duan, 1, 6Hailan Huang and 1Zonghua Wang
1College of Chemical and Environment Engineering, Qingdao University,
2Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China
3Beijing Centre for Physical and Chemical Analysis, Beijing, China
4Key Laboratory of Biofuels Materials, Shandong Provincial Key Laboratory of Energy Genetics Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China
5University of Chinese Academy of Sciences, Beijing, China
6Teachers College, Qingdao University, Qingdao, China
Advance Journal of Food Science and Technology  2015  11:882-885
http://dx.doi.org/10.19026/ajfst.9.1647  |  © The Author(s) 2015
Received: April ‎9, ‎2015   |  Accepted: May ‎10, ‎2015  |  Published: September 25, 2015
Back to issue  |  PDF   |   HTML

Abstract

A high performance anion-exchange chromatography with pulsed amperometric detection is developed for the determination of sucrose and glucosylglycerol in cyanobacterial cells. Under the optimized conditions, showed good linearity (r2>0.9999) with the chromatographic peak area in the range between 0.1 and 50.0 mg/L. The Limit Of Detection (LOD) is 0.15 mg/L for sucrose and 0.03 mg/L for glucosylglycerol. RSDs of the detection results are less than 2.0% (n = 8). The result showes that the method is convenient and accurate for the determination of sucrose and glucosylglycerol in cyanobacterial cells.

Keywords:

Anion exchange chromatography, cyanobacteria, glucosylglycerol, pulsed amperometric detection, sucrose,

References

  1. Badar-Goffer, R.S., H.S. Bachelard and P.G. Morris, 1990. Cerebral metabolism of acetate and glucose studied by 13C-nmr spectroscopy: A technique for investigating metabolic compartmentation in the brain [J]. Biochem. J., 266: 133-135.
    CrossRef    PMid:1968742 PMCid:PMC1131106    Direct Link
  2. Chen, S.F., M.G. Danao, V. Singh and P.J. Brown, 2014. Determining sucrose and glucose levels in dual-purpose sorghum stalks by Fourier transform near infrared (FT-NIR) spectroscopy [J]. J. Sci. Food Agr., 94(12): 2569-2576.
    CrossRef    PMid:24590962    Direct Link
  3. Gouda, M.D., M.A. Kumar and M.S. Thakur, 2002. Enhancement of operational stability of an enzyme biosensor for glucose and sucrose using protein based stabilizing agents [J]. Biosens. Bioelectron., 17(6-7): 503-505.
    CrossRef    Direct Link
  4. Kerem, I., B.I. Hakki and T. Ali, 2015. Rapid analysis of glucose, fructose and sucrose contents of commercial soft drinks using Raman spectroscopy [J]. Food Control, 48: 56-61.
    CrossRef    Direct Link
  5. Li, Y.Z., S.H. Liu, Z.Y. Zhu and Y.M. Yang, 2014. Determination of glucose, fructose and sucrose in jackfruit by HPLC [J]. Food Sci., 35: 84-87.
    Direct Link
  6. Liu, X., D. Li, J. Shi and C. Sun, 2010. Determination of monosaccharides and disaccharides in drink by ion chromatography with pulsed amperometric detector [J]. Chinese J. Spectrosc. Lab., 27: 441-445.
    Direct Link
  7. Malgorzata, G., L. Anna and S. Piotr, 2014. Simultaneous separation and determination of erythritol, xylitol, sorbitol, mannitol, maltitol, fructose, glucose, sucrose and maltose in food products by high performance liquid chromatography coupled to charged aerosol detector [J]. Microchem. J., 117: 77-82.
    CrossRef    Direct Link
  8. Mou, S. and Z. Li, 1995. The progress of Carbohydrates determination separation with pulsed am perometric by anion exchange dete ctiol [J]. Chinese J. Chromatogr., 13: 320-324.
  9. Pan, L., Q. Zhu and R. Lu, 2015. Determination of sucrose content in sugar beet by portable visible and near-infrared spectroscopy [J]. Food Chem., 167: 264-271.
    CrossRef    PMid:25148988    Direct Link
  10. Paul, L. and S. Jeffery, 2014. Assessment of crystallinity in processed sucrose by near- infrared spectroscopy and application to lyophiles [J]. J. Pharm. Sci., 103: 2884-2895.
    CrossRef    PMid:24824402    Direct Link
  11. Schoor, A., N. Erdmann, U. Effmert and S. Mikkat, 1995. Determination of the cyanobacterial osmolyte glucosylglycerol by high-performance liquid chromatography [J]. J. Chromatogr. A, 704: 89-97.
    CrossRef    Direct Link
  12. Sweeley, C.C., R. Bentley and M. Makita, 1963. Gas-liquid chromatography of trimethylsilyl derivatives of sugars and related substances [J]. J. Am. Chem. Soc., 85: 2497-2484.
    CrossRef    Direct Link
  13. Tang, K.T., L.N. Liang, Y.Q. Cai and S. Mou, 2007. Determination of sugars and sugar alcohols in tobacco feed liquids by high performance anion-exchange and pulsed amperometric Detection [J]. Chinese J. Anal. Chem., 25: 451-452.
    Direct Link
  14. Xie, Q., Q. Li, K. Wang and C. Liu, 2013. Determination of carbohydrate in honey and juhong pills by HPLC [J]. Chem. Anal. Meter., 1: 45-47.
    Direct Link

Competing interests

The authors have no competing interests.

Open Access Policy

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Copyright

The authors have no competing interests.
ISSN (Online):  2042-4876
ISSN (Print):   2042-4868
Submit Manuscript
   Information
   Sales & Services
Home   |  Contact us   |  About us   |  Privacy Policy
Copyright © 2024. MAXWELL Scientific Publication Corp., All rights reserved