Research Article | OPEN ACCESS
Determination of Sucrose and Glucosylglycerol in Intracellular Extracts of Cyanobacteria by Anion Exchange Chromatography
1, 2Xin Wang, 2Yun Fa, 3Shengfeng Le, 4, 5Yangkai Duan, 1, 6Hailan Huang and 1Zonghua Wang
1College of Chemical and Environment Engineering, Qingdao University,
2Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China
3Beijing Centre for Physical and Chemical Analysis, Beijing, China
4Key Laboratory of Biofuels Materials, Shandong Provincial Key Laboratory of Energy Genetics Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences,
Qingdao, China
5University of Chinese Academy of Sciences, Beijing, China
6Teachers College, Qingdao University, Qingdao, China
Advance Journal of Food Science and Technology 2015 11:882-885
Received: April 9, 2015 | Accepted: May 10, 2015 | Published: September 25, 2015
Abstract
A high performance anion-exchange chromatography with pulsed amperometric detection is developed for the determination of sucrose and glucosylglycerol in cyanobacterial cells. Under the optimized conditions, showed good linearity (r2>0.9999) with the chromatographic peak area in the range between 0.1 and 50.0 mg/L. The Limit Of Detection (LOD) is 0.15 mg/L for sucrose and 0.03 mg/L for glucosylglycerol. RSDs of the detection results are less than 2.0% (n = 8). The result showes that the method is convenient and accurate for the determination of sucrose and glucosylglycerol in cyanobacterial cells.
Keywords:
Anion exchange chromatography, cyanobacteria, glucosylglycerol, pulsed amperometric detection, sucrose,
References
-
Badar-Goffer, R.S., H.S. Bachelard and P.G. Morris, 1990. Cerebral metabolism of acetate and glucose studied by 13C-nmr spectroscopy: A technique for investigating metabolic compartmentation in the brain [J]. Biochem. J., 266: 133-135.
CrossRef PMid:1968742 PMCid:PMC1131106 Direct Link -
Chen, S.F., M.G. Danao, V. Singh and P.J. Brown, 2014. Determining sucrose and glucose levels in dual-purpose sorghum stalks by Fourier transform near infrared (FT-NIR) spectroscopy [J]. J. Sci. Food Agr., 94(12): 2569-2576.
CrossRef PMid:24590962 Direct Link -
Gouda, M.D., M.A. Kumar and M.S. Thakur, 2002. Enhancement of operational stability of an enzyme biosensor for glucose and sucrose using protein based stabilizing agents [J]. Biosens. Bioelectron., 17(6-7): 503-505.
CrossRef Direct Link -
Kerem, I., B.I. Hakki and T. Ali, 2015. Rapid analysis of glucose, fructose and sucrose contents of commercial soft drinks using Raman spectroscopy [J]. Food Control, 48: 56-61.
CrossRef Direct Link -
Li, Y.Z., S.H. Liu, Z.Y. Zhu and Y.M. Yang, 2014. Determination of glucose, fructose and sucrose in jackfruit by HPLC [J]. Food Sci., 35: 84-87.
Direct Link -
Liu, X., D. Li, J. Shi and C. Sun, 2010. Determination of monosaccharides and disaccharides in drink by ion chromatography with pulsed amperometric detector [J]. Chinese J. Spectrosc. Lab., 27: 441-445.
Direct Link -
Malgorzata, G., L. Anna and S. Piotr, 2014. Simultaneous separation and determination of erythritol, xylitol, sorbitol, mannitol, maltitol, fructose, glucose, sucrose and maltose in food products by high performance liquid chromatography coupled to charged aerosol detector [J]. Microchem. J., 117: 77-82.
CrossRef Direct Link -
Mou, S. and Z. Li, 1995. The progress of Carbohydrates determination separation with pulsed am perometric by anion exchange dete ctiol [J]. Chinese J. Chromatogr., 13: 320-324.
-
Pan, L., Q. Zhu and R. Lu, 2015. Determination of sucrose content in sugar beet by portable visible and near-infrared spectroscopy [J]. Food Chem., 167: 264-271.
CrossRef PMid:25148988 Direct Link -
Paul, L. and S. Jeffery, 2014. Assessment of crystallinity in processed sucrose by near- infrared spectroscopy and application to lyophiles [J]. J. Pharm. Sci., 103: 2884-2895.
CrossRef PMid:24824402 Direct Link -
Schoor, A., N. Erdmann, U. Effmert and S. Mikkat, 1995. Determination of the cyanobacterial osmolyte glucosylglycerol by high-performance liquid chromatography [J]. J. Chromatogr. A, 704: 89-97.
CrossRef Direct Link -
Sweeley, C.C., R. Bentley and M. Makita, 1963. Gas-liquid chromatography of trimethylsilyl derivatives of sugars and related substances [J]. J. Am. Chem. Soc., 85: 2497-2484.
CrossRef Direct Link -
Tang, K.T., L.N. Liang, Y.Q. Cai and S. Mou, 2007. Determination of sugars and sugar alcohols in tobacco feed liquids by high performance anion-exchange and pulsed amperometric Detection [J]. Chinese J. Anal. Chem., 25: 451-452.
Direct Link -
Xie, Q., Q. Li, K. Wang and C. Liu, 2013. Determination of carbohydrate in honey and juhong pills by HPLC [J]. Chem. Anal. Meter., 1: 45-47.
Direct Link
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
|
|
![](http://www.maxwellsci.com/images/AJFST-maxw.jpg) |
ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
![Submit Manuscript](../images/sub.jpg) |
Information |
|
|
|
Sales & Services |
|
|
|