Abstract

The aim of this study was to evaluate the potential use of whey components and lupin proteins as cheap nutrients in the formulation of optimum medium for lactic acid bacteria growth, for this purpose a Plackett and Burman design was used to screen variables (medium components and physical parameters) that have significant effects on the growth of Lactobacillus fermentum DSM 20049 in whey and lupin based medium. Statistical analysis of the results allowed selecting two factors having a significant positive effect on the bacterial growth (tween 80 and inoculum size). Central composite design was used to determine the optimum level of the selected factor. Under these optimized conditions, the composition of WLM (Whey and Lupin Medium) was the basic medium of whey and lupin supplemented with 1.56 mL/L of tween and inoculated at a rate of 5.25% with a maximum OD₆₀₀ value of 6.88. Based on the comparison of Lactobacillus fermentum DSM 20049 growth on the formulated medium with that observed on MRS (standard laboratory medium), the growth of the strain in WLM is much higher than that observed in MRS medium with a maximum OD₆₀₀ values of 7.04 on LWM and 4.08 on MRS medium, respectively and Lactobacillus fermentum DSM 20049 growth in the new medium was 72.54% higher than that on MRS. Thus, whey and lupin hydrolysates are good alternative sources of nitrogen that lead to a better growth of the strain and can be used for large-scale production.

Keywords:

Growth medium, Lactobacillus fermentum DSM 20049, lupin, optimization, whey,

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